sp. nov. and sp. nov., derived from human clinical disease and an emended description of Free

Abstract

Strains of members of the genus derived from ophthalmologic patients in Japan, Belgium and Switzerland and found to be closely related to-, but distinguishable from by 16S rRNA gene sequencing, were characterized using biochemical, chemotaxonomic, MALDI-TOF mass spectrometry and antimicrobial susceptibility methods and DNA–DNA hybridization as well as by whole-genome sequencing (WGS). Based on this investigation, we describe sp. nov. and sp. nov., derived from human ocular specimens, as well as emend the description of . Type strains for these species are: R-50085 (=LMG 28276 =CCUG 65815) and R-50187(=LMG 28277 =CCUG 65816). DNA G+C content was found to be 62.2 % (by HPLC) and 62.8 % (by WGS) for R-50085, 64.1 % (HPLC) and 64.8 % (WGS) for R-50187 and 67.8 % (HPLC) for LMG 19040 [=S-8 =CCUG 38654 =CECT 4843 =CIP 105509 =DSM 44356 =IFO (NBRC)16160 =JCM 12269].

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2016-08-01
2024-03-29
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References

  1. Bankevich A., Nurk S., Antipov D., Gurevich A. A., Dvorkin M., Kulikov A. S., Lesin V. M., Nikolenko S. I., Pham S. et al. 2012; SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477 [View Article][PubMed]
    [Google Scholar]
  2. Bernard K. A., Bellefeuille M., Ewan E. P. 1991; Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic Gram-positive rods. J Clin Microbiol 29:83–89[PubMed]
    [Google Scholar]
  3. Bernard K. A., Shuttleworth L., Munro C., Forbes-Faulkner J. C., Pitt D., Norton J. H., Thomas A. D. 2002a; Propionibacterium australiense sp. nov., derived from granulomatous bovine lesions. Anaerobe 8:41–47 [View Article]
    [Google Scholar]
  4. Bernard K. A., Munro C., Wiebe D., Ongsansoy E. 2002b; Characteristics of rare or recently-described Corynebacterium species recovered from human clinical material in Canada. J Clin Microbiol 40:4375–4381 [View Article]
    [Google Scholar]
  5. Bernard K. A., Funke G. 2012; Genus Corynebacterium. In Bergeys Manual of Systematic Bacteriology: The Actinobacteria, vol. 5 , pp. 245–289 Edited by Whitman W. B., Goodfellow M., Kämpfer P. New York: Springer;
    [Google Scholar]
  6. Boetzer M., Henkel C. V., Jansen H. J., Butler D., Pirovano W. 2011; Scaffolding pre-assembled contigs using SSPACE. Bioinformatics 27:578–579 [View Article][PubMed]
    [Google Scholar]
  7. Bruker. 2011 MALDI Biotyper 3.0 User Manual Revision 2 (February 2011) Bremens, Germany: Bruker Daltronic GmbH;
    [Google Scholar]
  8. Bruker Daltronics 2014; Bruker Custom MSP and Library Creation. Edited by: Anonymous Bruker
  9. Clinical Laboratory Standards Institute 2010 M45-2A. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guideline Number 18, 2nd edn. vol. 30 Wayne, PA: Clinical Laboratory Standards Institute;
    [Google Scholar]
  10. Collins M. D., Burton R. A., Jones D. 1988; Corynebacterium amycolatum sp. nov. a new mycolic acid-less Corynebacterium species from human skin. FEMS Microbiol Lett 49:349–352 [View Article]
    [Google Scholar]
  11. Collins M. D., Falsen E., Akervall E., Sjöden B., Alvarez A. 1998; Corynebacterium kroppenstedtii sp. nov., a novel corynebacterium that does not contain mycolic acids. Int J Syst Bacteriol 48:1449–1454 [View Article][PubMed]
    [Google Scholar]
  12. Collins M. D., Hoyles L., Foster G., Falsen E. 2004; Corynebacteriumcaspium sp. nov., from a caspian seal (Phoca caspica). Int J System Evol Microbiol 54:925–928 [View Article]
    [Google Scholar]
  13. Eguchi H., Kuwahara T., Miyamoto T., Nakayama-Imaohji H., Ichimura M., Hayashi T., Shiota H. 2008; High-level fluoroquinolone resistance in ophthalmic clinical isolates belonging to the species Corynebacterium macginleyi. J Clin Microbiol 46:527–532 [View Article][PubMed]
    [Google Scholar]
  14. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  15. Fernandez-Garayzabal J. F., Vela A. I., Egido R., Hutson R. A., Lanzarot M. P., Fernandez-Garcia M., Collins M. D. 2004; Corynebacterium ciconiae sp. nov., isolated from the trachea of black storks (Ciconia nigra). Int J of Evol Microbiol 54:2191–2195 [View Article]
    [Google Scholar]
  16. Fernandez-Garayzabal J. F., Collins M. D., Hutson R. A., Fernandez E., Monasterio R., Marco J., Dominguez L. 1997; Corynebacterium mastitidis sp. nov., isolated from milk of sheep with subclinical mastitis. Int J Syst Bacteriol 47:1082–1085 [View Article][PubMed]
    [Google Scholar]
  17. Goris J., Suzuki K., Vos P. D., Nakase T., Kersters K, Suzuki K. 1998; Evaluation of a microplate DNA–DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44:1148–1153 [View Article]
    [Google Scholar]
  18. Hall V., Collins M. D., Hutson R. A., Lawson P. A., Falsen E., Duerden B. 2003; Corynebacterium atypicum sp. nov., from a human clinical source, does not contain corynomycolic acids. Int J System Evol Microbiol 53:1065–1068 [View Article]
    [Google Scholar]
  19. Khamis A., Raoult D., La Scola B. 2004; rpoB gene sequencing for identification of Corynebacterium species. J Clin Microbiol 42:3925–3931 [View Article][PubMed]
    [Google Scholar]
  20. Magoč T., Salzberg S. L. 2011; FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27:2957–2963 [View Article][PubMed]
    [Google Scholar]
  21. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479:297–306 [View Article][PubMed]
    [Google Scholar]
  22. Richter M., Rosselló-Móra R. 2009; Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  23. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  24. Tippelt A., Albersmeier A., Brinkrolf K., Rückert C., Fernández-Natal I., Soriano F., Tauch A., Mollmann S., Jaenicke S. 2014; Mycolic acid biosynthesis genes in the genome sequence of Corynebacterium atypicum DSM 44849. Genome Announc 2: [View Article][PubMed]
    [Google Scholar]
  25. Wiertz R., Schulz S. C., Müller U., Kämpfer P., Lipski A. 2013; Corynebacterium frankenforstense sp. nov. and Corynebacterium lactis sp. nov., isolated from raw cow milk. Int J Syst Evol Microbiol 63:4495–4501 [View Article][PubMed]
    [Google Scholar]
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