1887

Abstract

Strains of members of the genus Corynebacterium derived from ophthalmologic patients in Japan, Belgium and Switzerland and found to be closely related to-, but distinguishable from Corynebacterium mastitidis by 16S rRNA gene sequencing, were characterized using biochemical, chemotaxonomic, MALDI-TOF mass spectrometry and antimicrobial susceptibility methods and DNA–DNA hybridization as well as by whole-genome sequencing (WGS). Based on this investigation, we describe Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human ocular specimens, as well as emend the description of Corynebacterium mastitidis . Type strains for these species are: C. lowii R-50085 (=LMG 28276 =CCUG 65815) and C. oculi R-50187(=LMG 28277 =CCUG 65816). DNA G+C content was found to be 62.2 % (by HPLC) and 62.8 % (by WGS) for C. lowii R-50085, 64.1 % (HPLC) and 64.8 % (WGS) for C. oculi R-50187 and 67.8 % (HPLC) for C. mastitidis LMG 19040 [=S-8 =CCUG 38654 =CECT 4843 =CIP 105509 =DSM 44356 =IFO (NBRC)16160 =JCM 12269].

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001059
2016-08-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/8/2803.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001059&mimeType=html&fmt=ahah

References

  1. Bankevich A., Nurk S., Antipov D., Gurevich A. A., Dvorkin M., Kulikov A. S., Lesin V. M., Nikolenko S. I., Pham S. et al.( 2012;). SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. . J Comput Biol 19: 455–477. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bernard K. A., Bellefeuille M., Ewan E. P..( 1991;). Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic Gram-positive rods. . J Clin Microbiol 29: 83–89.[PubMed]
    [Google Scholar]
  3. Bernard K. A., Shuttleworth L., Munro C., Forbes-Faulkner J. C., Pitt D., Norton J. H., Thomas A. D..( 2002a;). Propionibacterium australiense sp. nov., derived from granulomatous bovine lesions. . Anaerobe 8: 41–47. [CrossRef]
    [Google Scholar]
  4. Bernard K. A., Munro C., Wiebe D., Ongsansoy E..( 2002b;). Characteristics of rare or recently-described Corynebacterium species recovered from human clinical material in Canada. . J Clin Microbiol 40: 4375–4381. [CrossRef]
    [Google Scholar]
  5. Bernard K. A., Funke G..( 2012;). Genus Corynebacterium. . In Bergeys Manual of Systematic Bacteriology: The Actinobacteria, vol. 5 , pp. 245–289. Edited by Whitman W. B., Goodfellow M., Kämpfer P.. New York:: Springer;.
  6. Boetzer M., Henkel C. V., Jansen H. J., Butler D., Pirovano W..( 2011;). Scaffolding pre-assembled contigs using SSPACE. . Bioinformatics 27: 578–579. [CrossRef] [PubMed]
    [Google Scholar]
  7. Bruker.( 2011;). MALDI Biotyper 3.0 User Manual Revision 2 (February 2011). Bremens, Germany:: Bruker Daltronic GmbH;.
    [Google Scholar]
  8. Bruker Daltronics( 2014;). Bruker Custom MSP and Library Creation. . Edited by: Anonymous Bruker.
  9. Clinical Laboratory Standards Institute( 2010;). M45-2A. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guideline Number 18, , 2nd edn.,vol. 30. Wayne, PA:: Clinical Laboratory Standards Institute;.
    [Google Scholar]
  10. Collins M. D., Burton R. A., Jones D..( 1988;). Corynebacterium amycolatum sp. nov. a new mycolic acid-less Corynebacterium species from human skin. . FEMS Microbiol Lett 49: 349–352. [CrossRef]
    [Google Scholar]
  11. Collins M. D., Falsen E., Akervall E., Sjöden B., Alvarez A..( 1998;). Corynebacterium kroppenstedtii sp. nov., a novel corynebacterium that does not contain mycolic acids. . Int J Syst Bacteriol 48: 1449–1454. [CrossRef] [PubMed]
    [Google Scholar]
  12. Collins M. D., Hoyles L., Foster G., Falsen E..( 2004;). Corynebacteriumcaspium sp. nov., from a caspian seal (Phoca caspica). . Int J System Evol Microbiol 54: 925–928. [CrossRef]
    [Google Scholar]
  13. Eguchi H., Kuwahara T., Miyamoto T., Nakayama-Imaohji H., Ichimura M., Hayashi T., Shiota H..( 2008;). High-level fluoroquinolone resistance in ophthalmic clinical isolates belonging to the species Corynebacterium macginleyi. . J Clin Microbiol 46: 527–532. [CrossRef] [PubMed]
    [Google Scholar]
  14. Ezaki T., Hashimoto Y., Yabuuchi E..( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39: 224–229. [CrossRef]
    [Google Scholar]
  15. Fernandez-Garayzabal J. F., Vela A. I., Egido R., Hutson R. A., Lanzarot M. P., Fernandez-Garcia M., Collins M. D..( 2004;). Corynebacterium ciconiae sp. nov., isolated from the trachea of black storks (Ciconia nigra). . Int J of Evol Microbiol 54: 2191–2195. [CrossRef]
    [Google Scholar]
  16. Fernandez-Garayzabal J. F., Collins M. D., Hutson R. A., Fernandez E., Monasterio R., Marco J., Dominguez L..( 1997;). Corynebacterium mastitidis sp. nov., isolated from milk of sheep with subclinical mastitis. . Int J Syst Bacteriol 47: 1082–1085. [CrossRef] [PubMed]
    [Google Scholar]
  17. Goris J., Suzuki K., Vos P. D., Nakase T., Kersters K, Suzuki K..( 1998;). Evaluation of a microplate DNA–DNA hybridization method compared with the initial renaturation method. . Can J Microbiol 44: 1148–1153. [CrossRef]
    [Google Scholar]
  18. Hall V., Collins M. D., Hutson R. A., Lawson P. A., Falsen E., Duerden B..( 2003;). Corynebacterium atypicum sp. nov., from a human clinical source, does not contain corynomycolic acids. . Int J System Evol Microbiol 53: 1065–1068. [CrossRef]
    [Google Scholar]
  19. Khamis A., Raoult D., La Scola B..( 2004;). rpoB gene sequencing for identification of Corynebacterium species. . J Clin Microbiol 42: 3925–3931. [CrossRef] [PubMed]
    [Google Scholar]
  20. Magoč T., Salzberg S. L..( 2011;). FLASH: fast length adjustment of short reads to improve genome assemblies. . Bioinformatics 27: 2957–2963. [CrossRef] [PubMed]
    [Google Scholar]
  21. Mesbah M., Whitman W. B..( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr 479: 297–306. [CrossRef] [PubMed]
    [Google Scholar]
  22. Richter M., Rosselló-Móra R..( 2009;). Shifting the genomic gold standard for the prokaryotic species definition. . Proc Natl Acad Sci U S A 106: 19126–19131. [CrossRef] [PubMed]
    [Google Scholar]
  23. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). MEGA6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tippelt A., Albersmeier A., Brinkrolf K., Rückert C., Fernández-Natal I., Soriano F., Tauch A., Mollmann S., Jaenicke S..( 2014;). Mycolic acid biosynthesis genes in the genome sequence of Corynebacterium atypicum DSM 44849. . Genome Announc 2:. [CrossRef] [PubMed]
    [Google Scholar]
  25. Wiertz R., Schulz S. C., Müller U., Kämpfer P., Lipski A..( 2013;). Corynebacterium frankenforstense sp. nov. and Corynebacterium lactis sp. nov., isolated from raw cow milk. . Int J Syst Evol Microbiol 63: 4495–4501. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001059
Loading
/content/journal/ijsem/10.1099/ijsem.0.001059
Loading

Data & Media loading...

Supplements

Supplementary File 1



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error