RT Journal Article SR Electronic(1) A1 Tanasupawat, Somboon A1 Takehana, Toshihiko A1 Yoshida, Shosuke A1 Hiraga, Kazumi A1 Oda, KoheiYR 2016 T1 Ideonella sakaiensis sp. nov., isolated from a microbial consortium that degrades poly(ethylene terephthalate) JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 8 SP 2813 OP 2818 DO https://doi.org/10.1099/ijsem.0.001058 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, aerobic, non-spore-forming, rod-shaped bacterium, designed strain 201-F6T, was isolated from a microbial consortium that degrades poly(ethylene terephthalate) (PET) collected in Sakai city, Japan, and was characterized on the basis of a polyphasic taxonomic study. The cells were motile with a polar flagellum. The strain contained cytochrome oxidase and catalase. It grew within the pH range 5.5–9.0 (optimally at pH 7–7.5) and at 15–42 ºC (optimally at 30–37 ºC). The major isoprenoid quinone was ubiquinone with eight isoprene units (Q-8). C16 : 0, C1 7 : 0 cyclo, C18 :1ω7c and C12 : 0 2-OH were the predominant cellular fatty acids. The major polar lipids were phosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of genomic DNA was 70.4 mol%. Phylogenetic analysis using the 16S rRNA gene sequences showed that strain 201-F6T was affiliated to the genus Ideonella , and was closely related to Ideonella dechloratans LMG 28178T (97.7 %) and Ideonella azotifigens JCM 15503T (96.6 %). Strain 201-F6T could be clearly distinguished from the related species of the genus Ideonella by its physiological and biochemical characteristics as well as by its phylogenetic position and DNA–DNA relatedness. Therefore, the strain represents a novel species of the genus Ideonella , for which the name Ideonella sakaiensis sp. nov. (type strain 201-F6T=NBRC 110686T=TISTR 2288T) is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001058