%0 Journal Article %A Oshkin, Igor Y. %A Belova, Svetlana E. %A Danilova, Olga V. %A Miroshnikov, Kirill K. %A Rijpstra, W. Irene C. %A Sinninghe Damsté, Jaap S. %A Liesack, Werner %A Dedysh, Svetlana N. %T Methylovulum psychrotolerans sp. nov., a cold-adapted methanotroph from low-temperature terrestrial environments, and emended description of the genus Methylovulum %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 6 %P 2417-2423 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001046 %K West Siberian methane seeps %K subarctic freshwater lakes %K methane oxidation at low temperatures %K cold-adapted methanotrophs %K Methylovulum psychrotolerans sp. nov. %I Microbiology Society, %X Two isolates of aerobic methanotrophic bacteria, strains Sph1T and Sph2, were obtained from cold methane seeps in a floodplain of the river Mukhrinskaya, Irtysh basin, West Siberia. Another morphologically and phenotypically similar methanotroph, strain OZ2, was isolated from a sediment of a subarctic freshwater lake, Archangelsk region, northern Russia. Cells of these three strains were Gram-stain-negative, light-pink-pigmented, non-motile, encapsulated, large cocci that contained an intracytoplasmic membrane system typical of type I methanotrophs. They possessed a particulate methane monooxygenase enzyme and utilized only methane and methanol. Strains Sph1T, Sph2 and OZ2 were able to grow at a pH range of 4.0–8.9 (optimum at pH 6.0–7.0) and at temperatures between 2 and 36 °C. Although their temperature optimum was at 20–25 °C, these methanotrophs grew well at lower temperatures, down to 4 °C. The major cellular fatty acids were C16 : 1ω5c, C16 : 1ω6c, C16 : 1ω7c, C16 : 1ω8c, C16 : 0 and C14 : 0; the DNA G+C content was 51.4–51.9 mol%. Strains Sph1T, Sph2 and OZ2 displayed nearly identical (99.1–99.7 % similarity) 16S rRNA gene sequences and belonged to the family Methylococcaceae of the class Gammaproteobacteria . The most closely related organism was Methylovulum miyakonense HT12T (96.0–96.5 % 16S rRNA gene sequence similarity and 90 % pmoA sequence similarity). The novel isolates, however, differed from Methylovulum miyakonense HT12T by cell morphology, pigmentation, absence of soluble methane monooxygenase, more active growth at low temperatures, growth over a broader pH range and higher DNA G+C content. On the basis of these differences, we propose a novel species, Methylovulum psychrotolerans sp. nov., to accommodate these methanotrophs. Strain Sph1T (=LMG 29227T=VKM B-3018T) is the type strain. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001046