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Abstract

Two novel actinobacteria, designated strains NEAU-Jh1-4 and NEAU-Wp2-0, were isolated from muddy soil collected from a riverbank in Jiaohe and a dandelion root collected from Harbin, respectively. A polyphasic study was carried out to establish the taxonomic positions of these two strains. The phylogenetic analysis based on the 16S rRNA gene sequences of strains NEAU-Jh1-4 and NEAU-Wp2-0 indicated that strain NEAU-Jh1-4 clustered with NEAU-NH11 (99.32 % 16S rRNA gene sequence similarity), CY-15110(98.30 %) and se CY-11007 (97.95 %) and strain NEAU-Wp2-0clustered with ‘’ NEAU-QS7 (99.39 %), ‘’ NEAU-ML10 (99.26 %), ‘’ NEAU-GH7 (98.85 %) and DSM 43180 (98.69 %). Moreover, morphological and chemotaxonomic properties of the two isolates also confirmed their affiliation to the genus . However, the low level of DNA–DNA hybridization and some phenotypic characteristics allowed the isolates to be differentiated from the most closely related species. Therefore, it is proposed that strains NEAU-Jh1-4 and NEAU-Wp2-0 represent two novel species of the genus , for which the name sp. nov. and sp. nov. are proposed. The type strains are NEAU-Jh1-4 (=CGMCC 4.7213=JCM 30348) and NEAU-Wp2-0 (=CGMCC 4.7217=JCM 30349), respectively.

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2016-06-10
2020-04-07
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