RT Journal Article SR Electronic(1) A1 Sun, Le-Ni A1 Wang, Dao-Sheng A1 Yang, En-Dong A1 Fang, Lian-Cheng A1 Chen, Yi-Fei A1 Tang, Xin-Yun A1 Hua, Ri-MaoYR 2016 T1 Cupriavidus nantongensis sp. nov., a novel chlorpyrifos-degrading bacterium isolated from sludge JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 6 SP 2335 OP 2341 DO https://doi.org/10.1099/ijsem.0.001034 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, aerobic, coccoid to small rod-shaped bacterium, designated X1T, was isolated from sludge collected from the vicinity of a pesticide manufacturer in Nantong, Jiangsu Province, China. Based on 16S rRNA gene sequence analysis, strain X1T belonged to the genus Cupriavidus , and was most closely related to Cupriavidus taiwanensis LMG 19424T (99.1 % 16S rRNA gene sequence similarity) and Cupriavidus alkaliphilus LMG 26294T (98.9 %). Strain X1T showed 16S rRNA gene sequence similarities of 97.2–98.2 % with other species of the genus Cupriavidus . The major cellular fatty acids of strain X1T were C16 : 0, C16 : 1ω7c and/or iso-C15 : 0 2-OH (summed feature 3), C18 : 1ω7c and C17 : 0 cyclo, and the major respiratory quinone was ubiquinone Q-8. The major polar lipids of strain X1T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminophospholipid, phospholipid and hydroxyphosphatidylethanolamine. The DNA G+C content was 66.6 mol%. The DNA–DNA relatedness values of strain X1T with the five reference strains C. taiwanensis LMG 19424T, C. alkaliphilus LMG 26294T, Cupriavidus necator LMG 8453T, Cupriavidus gilardii LMG 5886T and ‘ Cupriavidus yeoncheonense ' KCTC 42053 were lower than 70 %. The results obtained from phylogenetic analysis, phenotypic characterization and DNA–DNA hybridization indicated that strain X1T should be proposed to represent a novel species of the genus Cupriavidus , for which the name Cupriavidus nantongensis sp. nov. is proposed. The type strain is X1T (=KCTC 42909T=LMG 29218T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001034