1887

Abstract

Two extremely halophilic archaeal strains, DYS4 and Y2, were isolated from rock salt of the Jiangcheng Salt Mine, Yunnan province, China. Cells of the two strains were non-motile, pleomorphic rods and Gram-stain-negative. The cells produced light red-pigmented colonies. Strains DYS4 and Y2 required 2.6-3.4 M NaCl, pH 7.5– 8.0 and 42 ºC in aerobic conditions for optimal growth. Mgwas required for growth. The major polar lipids of both strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. An unidentified minor glycolipid spot was present for strains DYS4 and Y2, which differentiates them from the closely related species of the genera and . The lipid core of the glycolipid was -2,3-diphytanylglycerol (CC). The sequence similarity of the 16S rRNA gene demonstrated that the closest relatives of strains DYS4 and Y2 were 31-hong (94.1 % and 93.6 % 16S rRNA gene sequence similarity to DYS4 and Y2, respectively) and SKJ47 (93.4% and 93.1%). Phylogenetic analysis of the 16S rRNA gene and the gene revealed that strains DYS4 and Y2 formed an independent lineage closely related to the genera and . The DNA G+C contents of strains DYS4 and Y2 were 68.2 and 67.0 mol%, respectively. The DNA–DNA relatedness value between strains DYS4 and Y2 was 90.0 ± 0.5%, while that between strain DYS4 and other closest relatives was less than 26 % (19 ± 0.7 % for 31-hong and 25 ± 0.3% for SKJ47). The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains DYS4 and Y2 (=CGMCC 1.15000=JCM 30892) represent a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is DYS4 (=CGMCC 1.14998=JCM 30891).

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2016-06-10
2021-10-28
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