@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001028, author = "Zhang, Shanshan and Li, Zhaojun and Yan, Yanchun and Zhang, Chuanlun and Li, Jun and Zhao, Baisuo", title = "Bacillus urumqiensis sp. nov., a moderately haloalkaliphilic bacterium isolated from a salt lake", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "6", pages = "2305-2312", doi = "https://doi.org/10.1099/ijsem.0.001028", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001028", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Bacillus urumqiensis", keywords = "Xinjiang Uyghur Autonomous Region", keywords = "salt lake", keywords = "moderately halophilic alkaliphilic", abstract = "A Gram-stain-positive, rod-shaped, aerobic and moderately haloalkaliphilic bacterium, designated BZ-SZ-XJ18T, was isolated from the mixed water and sediment of a saline-alkaline lake located in the Xinjiang Uyghur Autonomous Region of China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BZ-SZ-XJ18T was a member of the genus Bacillus . The closest phylogenetic relatives were Bacillus saliphilus 6AGT (96.7 % 16S rRNA gene sequence similarity), ‘ Bacillus daqingensis’ X10-1 (96.6 %), Bacillus luteus JC167T (96.5 %), Bacillus daliensis DLS13T (96.2 %), Bacillus chagannorensis CG-15T (95.2 %) and Bacillus polygoni YN-1T (95.0 %). DNA–DNA relatedness between strain BZ-SZ-XJ18T and the reference type strains of the related species of the genus Bacillus was lower than 27 %. The isolate formed yellow pigment and grew in the presence of 0.22–4.32 M Na+ (equivalent to 1.3–25.3 %, w/v, NaCl) (optimum 1.08 M Na+, equivalent to 6.3 %, w/v, NaCl), at pH 6.5–10.0 (optimum pH 8.5–9.5) and at 8–41 ºC (optimum 37 ºC). The major cellular fatty acids were anteiso-C15:0 (43.0 %), C16:0 (18.1 %), iso-C15:0 (11.3 %), anteiso-C17:0 (8.0 %) and iso-C16:0 (7.0 %). The major polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol. The main respiratory quinone was menaquinone-7 (MK-7), and the peptidoglycan type of the cell wall was A1γ based on meso-diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 42.3 mol% (HPLC) or 41.4 mol% (T m). On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain BZ-SZ-XJ18T is proposed to represent a novel species, Bacillus urumqiensis within the genus Bacillus . The type strain is BZ-SZ-XJ18T (=DSM 29145T=JCM 30195T).", }