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Volume 66, Issue 6

sp. nov., a moderately haloalkaliphilic bacterium isolated from a salt lake Free

Abstract

A Gram-stain-positive, rod-shaped, aerobic and moderately haloalkaliphilic bacterium, designated BZ-SZ-XJ18, was isolated from the mixed water and sediment of a saline-alkaline lake located in the Xinjiang Uyghur Autonomous Region of China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BZ-SZ-XJ18 was a member of the genus . The closest phylogenetic relatives were 6AG (96.7 % 16S rRNA gene sequence similarity), ‘’ X10-1 (96.6 %), JC167 (96.5 %), DLS13 (96.2 %), CG-15 (95.2 %) and YN-1 (95.0 %). DNA–DNA relatedness between strain BZ-SZ-XJ18 and the reference type strains of the related species of the genus was lower than 27 %. The isolate formed yellow pigment and grew in the presence of 0.22–4.32 M Na (equivalent to 1.3–25.3 %, w/v, NaCl) (optimum 1.08 M Na, equivalent to 6.3 %, w/v, NaCl), at pH 6.5–10.0 (optimum pH 8.5–9.5) and at 8–41 ºC (optimum 37 ºC). The major cellular fatty acids were anteiso-C (43.0 %), C (18.1 %), iso-C (11.3 %), anteiso-C (8.0 %) and iso-C (7.0 %). The major polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol. The main respiratory quinone was menaquinone-7 (MK-7), and the peptidoglycan type of the cell wall was A1γ based on -diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 42.3 mol% (HPLC) or 41.4 mol% ( ). On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain BZ-SZ-XJ18 is proposed to represent a novel species, within the genus . The type strain is BZ-SZ-XJ18 (=DSM 29145=JCM 30195).

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  • Accepted:
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Keyword(s): Bacillus urumqiensis , moderately halophilic alkaliphilic , salt lake and Xinjiang Uyghur Autonomous Region
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2016-06-10
2024-04-16
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Bacillus urumqiensis sp. nov., a moderately haloalkaliphilic bacterium isolated from a salt lake
Int J Syst Evol Microbiol 66, 2305 (2016); https://doi.org/10.1099/ijsem.0.001028
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