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Volume 66, Issue 6

gen. nov., sp. nov., an -acyl-homoserine-lactone-producing bacterium in the family isolated from landfill site soil Free

Abstract

Phylogenetic and taxonomic characterization was performed for bacterium RB-25, which was isolated from a soil sample collected in a former municipal landfill site in Puchong, Malaysia. Growth occurred at 20–37 °C at pH 5–8 but not in the presence of 9 % (w/v) NaCl or higher. The principal fatty acids were C, Cω7 and summed feature 3 (Cω7 and/or iso-C 2-OH). Ubiquinone-8 was the only isoprenoid quinone detected. Polar lipid analysis revealed the presence of phospholipid, phosphoaminolipid, phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. DNA G+C content was 50.9 mol% phylogenetic analysis based on 16S rRNA gene sequence showed that strain RB-25 formed a distinct lineage within the family of the class . It exhibited a low level of 16S rRNA gene sequence similarity with its phylogenetic neighbours LMG 26275(96.6 %), CIP 78.65 (96.5 %), ATCC 43762(96.4 %), LMG 26273 (96.3 %), NUM 1720(96.3 %) and C1 (96.2 %). Multilocus sequence analyses based on and sequences showed a clear distinction of strain RB-25 from the most closely related genera. Isolate RB-25 could also be distinguished from members of these genera by a combination of the DNA G+C content, respiratory quinone system, fatty acid profile, polar lipid composition and other phenotypic features. Strain RB-25 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is RB-25 (=DSM 28811=LMG 28304).

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  • Accepted:
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Keyword(s): Chitinase operon , Complete genome sequence , Dumping ground , Multilocus sequence analyses , Polyphasic identification , Quorum sensing and RpoB gene
© 2016 IUMS
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2016-06-10
2024-04-19
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Chania multitudinisentens gen. nov., sp. nov., an N-acyl-homoserine-lactone-producing bacterium in the family Enterobacteriaceae isolated from landfill site soil
Int J Syst Evol Microbiol 66, 2297 (2016); https://doi.org/10.1099/ijsem.0.001025
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