1887

Abstract

An endophytic actinobacterium, strain PIP 158, was isolated from the stem of a native apricot tree () collected from the grounds of Flinders University, Adelaide, Australia. As a result of a polyphasic taxonomic study, this strain was identified as a member of the genus . This strain was a Gram-stain-positive, aerobic actinobacterium with well-developed substrate mycelia which were non-motile and with hyphae fragmenting into short to elongated rod-like elements. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed this isolate in the family being most closely related to ATCC 39419and BC640which share a similarity of 99. 26 and 99.18 % with S1.4, respectively. Chemotaxonomic data including cell-wall components, major menaquinones and major fatty acids confirmed the affiliation of strain PIP 158to the genus The results of the phylogenetic analysis, including physiological and biochemical studies in combination with DNA–DNA hybridization, allowed the genotypic and phenotypic differentiation of strain PIP 158from the closest related species with validly published names. The name proposed for the novel species is sp. nov. The type strain is PIP 158 (=DSM 23717=NRRL B-24813).

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2016-06-10
2020-01-23
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