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Abstract

A Gram-stain-negative, coccoid- or oval-shaped and non-motile bacterial strain, designated MM-10, belonging to the Alphaproteobacteria , was isolated from seawater of the Yellow Sea, Korea, and was subjected to a polyphasic taxonomic study. Strain MM-10 grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain MM-10 fell within the clade comprising members of the genera Mameliella, Sagittula, Antarctobacter , , and Ponticoccus . Strain MM-10 exhibited a 16S rRNA gene sequence similarity of 97.7 % with respect to the type strain of Mameliella alba . The DNA G+C content of strain MM-10 was 66.0 mol% and its mean level of DNA–DNA relatedness with M. alba LMG 24665 was 12.3±1.4 % . Strain MM-10 contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrate that strain MM-10 is distinguishable from recognized species of the genera Mameliella, Sagittula , and Antarctobacter . On the basis of the data presented, strain MM-10 is considered to represent a novel species of a new genus, for which the name Maliponia aquimaris gen. nov., sp. nov. is proposed. The type strain of Maliponia aquimaris is MM-10 (=KCTC 42721=CECT 8898).

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2016-06-10
2019-12-06
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