1887

Abstract

A novel actinobacterium, designated strain NEAU-ND5, was isolated from the rhizosphere of corn ( L.) collected in Heilongjiang Province, north-east China, and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain NEAU-ND5 was a member of the genus , with highest sequence similarities to A4036 (98.29 %), GW12687 (98.25 %), HMC10 (98.22 %), YIM 67092 (98.04 %) and NRRL B-24325 (98.04 %). Similarities to other type strains of the genus were lower than 98 %. Morphological and chemotaxonomic properties of strain NEAU-ND5 were also consistent with the description of the genus . The cell wall contained -diaminopimelic acid and the whole-cell sugars were glucose, ribose and madurose. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major menaquinones were MK-9(H), MK-9(H) and MK-9(H). The predominant cellular fatty acids were iso-C and 10-methyl C. A combination of DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-ND5 was clearly distinguished from its closely related species. Consequently, it is concluded that strain NEAU-ND5 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is NEAU-ND5 (=CGMCC 4.7280=DSM 100528).

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2016-06-10
2020-01-26
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References

  1. Atlas R. M.. 2004; Handbook of Microbiological Media, 3rd edn Boca Raton, FL: CRC Press;[CrossRef]
    [Google Scholar]
  2. Chen S., Shi J., Li D., Wu Y., Huang Y.. 2015; Nonomuraea flavida sp. nov., a novel species of soil actinomycete isolated from Aconitum napellus rhizosphere. Int J Syst Evol Microbiol65:4055–4060[CrossRef]
    [Google Scholar]
  3. Chiba S., Suzuki M., Ando K.. 1999; Taxonomic re-evaluation of ‘Nocardiopsis' sp. K-252T (= NRRL 15532T): a proposal to transfer this strain to the genus Nonomuraea as Nonomuraea longicatena sp. nov. Int J Syst Bacteriol49:1623–1630 [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M. D.. 1985; Isoprenoid quinone analyses in bacterial classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–284 Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A.. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–789 [CrossRef]
    [Google Scholar]
  8. Gao R. X., Liu C. X., Zhao J. W., Jia F. Y., Yu C., Yang L. Y., Wang X. J., Xiang W. S.. 2014; Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora . Antonie Van Leeuwenhoek105:307–315 [CrossRef][PubMed]
    [Google Scholar]
  9. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N.. 1974; Nocardia coeliaca,Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol24:54–63 [CrossRef]
    [Google Scholar]
  10. Hayakawa M., Nonomura H.. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol65:501–509[CrossRef]
    [Google Scholar]
  11. Huss V. A. R., Festl H., Schleifer K. H.. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol4:184–192 [CrossRef][PubMed]
    [Google Scholar]
  12. Jia F. Y., Liu C. X., Wang X. J., Zhao J. W., Liu Q. F., Zhang J., Gao R. X., Xiang W. S.. 2013; Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae . Antonie van Leeuwenhoek103:399–408 [CrossRef][PubMed]
    [Google Scholar]
  13. Jia F. Y., Liu C. X., Zhao J. W., Zhang Y. J., Li L. J., Zhou S. Y., Shen Y., Wang X. J., Xiang W.. 2015; Streptomyces vulcanius sp. nov., a novel actinomycete isolated from volcanic sediment. Antonie van Leeuwenhoek107:15–21 [CrossRef][PubMed]
    [Google Scholar]
  14. Kelly K. L.. 1964; Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  16. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120[PubMed][CrossRef]
    [Google Scholar]
  17. Kroppenstedt R. M.. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics, pp. 173–199 Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;
    [Google Scholar]
  18. Lechevalier M. P., Lechevalier H. A.. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol20:435–443[CrossRef]
    [Google Scholar]
  19. Lechevalier M. P., Lechevalier H. A.. 1980; The Chemotaxonomy of Actinomycetes. In Actinomycete Taxonomy, Special Publication pp.277–284 Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  20. Lechevalier M. P., De Bièvre C., Lechevalier H.. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol5:249–260[CrossRef]
    [Google Scholar]
  21. Mandel M., Marmur J.. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol12:195–206 [CrossRef]
    [Google Scholar]
  22. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J.. 2000; A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of gram-positive bacteria. Lett Appl Microbiol30:178–182[PubMed][CrossRef]
    [Google Scholar]
  23. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M.. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr188:221–233[CrossRef]
    [Google Scholar]
  24. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241[CrossRef]
    [Google Scholar]
  25. Nonomura H., Ohara Y.. 1971; Distribution of actinomycetes in soil XI. Some new species of the genus Actinomadura Lechevalier et al . J Ferment Technol49:904–912
    [Google Scholar]
  26. Quadri S. R., Tian X. P., Zhang J., Li J., Nie G. X., Tang S. K., Al Ruwaili J., Agsar D., Li W. J., Dastager S. G.. 2015; Nonomuraea indica sp. nov., novel actinomycetes isolated from limestone open pit mine, India. J Antibiot68:491–495 [CrossRef][PubMed]
    [Google Scholar]
  27. Rachniyom H., Matsumoto A., Indananda C., Duangmal K., Takahashi Y., Thamchaipenet A.. 2015; Nonomuraea syzygi sp. nov., an endophytic actinomycete isolated from the roots of a jambolan plum tree (Syzygium cumini L. Skeels). Int J Syst Evol Microbiol65:1234–1240 [CrossRef][PubMed]
    [Google Scholar]
  28. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  29. Shirling E. B., Gottlieb D.. 1966; Methods for characterization of Streptomyces species. Int Syst Bacteriol16:313–340[CrossRef]
    [Google Scholar]
  30. Smibert R. M., Krieg N. R.. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  31. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  32. Uchida K., Kudo T., Suzuki K., Nakase T.. 1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol45:49–56 [CrossRef][PubMed]
    [Google Scholar]
  33. Waksman S. A.. 1961; The Actinomycetes, Classification, Identification and Descriptions of Genera and Species, vol. 2 Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  34. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464[CrossRef]
    [Google Scholar]
  35. Wu C., Lu X., Qin M., Wang Y., Ruan J.. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology16:176–178
    [Google Scholar]
  36. Xiang W. S., Liu C. X., Wang X. J., Du J., Xi L. J., Huang Y.. 2011; Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). Int J Syst Evol Microbiol61:1165–1169 [CrossRef][PubMed]
    [Google Scholar]
  37. Xie Q. Y., Lin H. P., Li L., Brown R., Goodfellow M., Deng Z. X., Hong K.. 2012; Verrucosispora wenchangensis sp. nov., isolated from mangrove soil. Antonie Van Leeuwenhoek102:1–7 [CrossRef][PubMed]
    [Google Scholar]
  38. Yokota A., Tamura T., Hasegawa T., Huang L. H.. 1993; Catenuloplanes japonicas gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . Int J Syst Bacteriol43:805–812 [CrossRef]
    [Google Scholar]
  39. Zhang Z., Wang Y., Ruan J.. 1998; Reclassification of Thermomonospora and Microtetraspora . Int J Syst Bacteriol48:411–422 [CrossRef][PubMed]
    [Google Scholar]
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