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Abstract

A novel gliding bacterial strain, GB 56.1, was obtained from soil at the Sakaerat Biosphere Reserve, in Nakhon Ratchasima province, Thailand; the strain was characterized using a polyphasic approach. Cells were Gram-stain-negative, yellow, rod shaped and devoid of flagella, but showed gliding motility. Phylogenetic analysis based on 16S rRNA gene sequences found that GB 56.1 was a member of the genus and that the strain shared the highest sequence similarities with (98.4 %), (98.3 %) and (97.9 %). The similarities of the sequences of all other species of the genus were below 97.4 %. The major respiratory quinone of strain GB 56.1 was MK-6; fatty acids were iso-C, Cω6/Cω7, C and C 3-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylserine, an unidentified amino lipid and four polar lipids. The DNA G+C content of this strain was 34.2 mol%. The DNA–DNA relatedness of GB 56.1was highest against with a value of 37.6 %. On the basis of morphological, physiological and chemotaxonomic characteristics, DNA–DNA hybridization relatedness and 16S rRNA gene sequence analysis, we conclude that strain GB 56.1 represents a novel species, for which the name sp. nov. is proposed. The type strain is GB 56.1 (=TISTR 1612 =KCTC 42679).

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2016-06-10
2020-01-22
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