1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped bacterium motile by means of a single polar flagella, strain ST-6, was isolated from a brown alga () collected in Jeju, Republic of Korea. Strain ST-6 was psychrotolerant, growing at 4–30 °C (optimum 20 °C). Phylogenetic analysis based on 16S rRNA and gene sequences revealed that strain ST-6 belonged to a distinct lineage in the genus . Strain ST-6was related most closely to J83, TF-27, IT12, M7 and SC18, showing 96–97 % and 85–70 % 16S rRNA and gene sequences similarities, respectively. DNA–DNA relatedness values between strain ST-6 and the type strains of two species of the genus were <22.6 %. The major cellular fatty acids (>5 %) were summed feature 3 (comprising Cω7 and/ or iso-C2-OH), C, iso-C and Cω8. The DNA G+C content of strain ST-6was 42.4 mol%, and the predominant isoprenoid quinones were menaquinone MK-7 and ubiquinones Q-7 and Q-8. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain ST-6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ST-6 (= KCTC 23253 = JCM 31091).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001014
2016-06-10
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/6/2218.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001014&mimeType=html&fmt=ahah

References

  1. Bowman J. P.. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol50:1861–1868 [CrossRef][PubMed]
    [Google Scholar]
  2. Bowman J. P., McCammon S. A., Nichols D. S., Skerratt J. H., Rea S. M., Nichols P. D., McMeekin T. A.. 1997; Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20:5 omega 3) and grow anaerobically by dissimilatory Fe(III) reduction. Int J Syst Bacteriol47:1040–1047 [CrossRef][PubMed]
    [Google Scholar]
  3. Bozal N., Montes M. J., Miñana-Galbis D., Manresa A., Mercadé E.. 2009; Shewanella vesiculosa sp. nov., a psychrotolerant bacterium isolated from an Antarctic coastal area. Int J Syst Evol Microbiol59:336–340 [CrossRef][PubMed]
    [Google Scholar]
  4. Bozal N., Montes M. J., Tudela E., Jiménez F., Guinea J.. 2002; Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol52:195–205 [CrossRef][PubMed]
    [Google Scholar]
  5. Brettar I., Christen R., Höfle M. G.. 2002; Shewanella denitrificans sp. nov., a vigorously denitrifying bacterium isolated from the oxic-anoxic interface of the gotland deep in the central Baltic Sea. Int J Syst Evol Microbiol52:2211–2217 [CrossRef][PubMed]
    [Google Scholar]
  6. Brink A. J., van Straten A., van Rensburg A. J.. 1995; Shewanella (Pseudomonas) putrefaciens bacteremia. Clin Infect Dis20:1327–1332[PubMed][CrossRef]
    [Google Scholar]
  7. Bruns A., Rohde M., Berthe-Corti L.. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol51:1997–2006 [CrossRef][PubMed]
    [Google Scholar]
  8. Chang H. W., Roh S. W., Kim K. H., Nam Y. D., Jeon C. O., Oh H. M., Bae J. W.. 2008; Shewanella basaltis sp. nov., a marine bacterium isolated from black sand. Int J Syst Evol Microbiol58:1907–1010 [CrossRef][PubMed]
    [Google Scholar]
  9. Debois J., Degreef H., Vandepitte J., Spaepen J.. 1975; Pseudomonas putrefaciens as a cause of infection in humans. J Clin Pathol28:993–996[PubMed][CrossRef]
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E.. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39:224–229[CrossRef]
    [Google Scholar]
  11. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: A maximum likelihood approach. J Mol Evol17:368–376[PubMed][CrossRef]
    [Google Scholar]
  12. Felsenstein J.. 1985; Confidence limits on phylogenies: An approach using the bootstrap. Evolution39:783–791[CrossRef]
    [Google Scholar]
  13. Gao H., Obraztova A., Stewart N., Popa R., Fredrickson J. K., Tiedje J. M., Nealson K. H., Zhou J.. 2006; Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean. Int J Syst Evol Microbiol56:1911–1916 [CrossRef][PubMed]
    [Google Scholar]
  14. Hall T. A.. 1999; Bioedit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser41:95–98
    [Google Scholar]
  15. Holmes B., Lapage S. P., Malnick H.. 1975; Strains of pseudomonas putrefaciens from clinical material. J Clin Pathol28:149–155[PubMed][CrossRef]
    [Google Scholar]
  16. Ivanova E. P., Flavier S., Christen R.. 2004a; Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol54:1773–1788[CrossRef]
    [Google Scholar]
  17. Ivanova E. P., Nedashkovskaya O. I., Sawabe T., Zhukova N. V., Frolova G. M., Nicolau D. V., Mikhailov V. V., Bowman J. P.. 2004b; Shewanella affinis sp. nov., isolated from marine invertebrates. Int J Syst Evol Microbiol54:1089–1093[CrossRef]
    [Google Scholar]
  18. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing eztaxon-e: A prokaryotic 16S rrna gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  19. Kim S. J., Park S. J., Oh Y. S., Lee S. A., Shin K. S., Roh D. H., Rhee S. K.. 2012; Shewanella arctica sp. nov., an iron-reducing bacterium isolated from Arctic marine sediment. Int J Syst Evol Microbiol62:1128–1133 [CrossRef][PubMed]
    [Google Scholar]
  20. Kluge A. G., Farris F. S.. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool18:1–32[CrossRef]
    [Google Scholar]
  21. Komagata K., Suzuki K.. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol19:161–207[CrossRef]
    [Google Scholar]
  22. Lee M. H., Yoon J. H.. 2012; Shewanella litorisediminis sp. nov., a gammaproteobacterium isolated from a tidal flat sediment. Antonie Van Leeuwenhoek102:591–599 [CrossRef][PubMed]
    [Google Scholar]
  23. Lee O. O., Lau S. C., Tsoi M. M., Li X., Plakhotnikova I., Dobretsov S., Wu M. C., Wong P. K., Weinbauer M. et al. 2006; Shewanella irciniae sp. nov., a novel member of the family Shewanellaceae, isolated from the marine sponge Ircinia dendroides in the Bay of Villefranche, Mediterranean Sea. Int J Syst Evol Microbiol56:2871–2877 [CrossRef][PubMed]
    [Google Scholar]
  24. MacDonell M. T., Colwell R. R.. 1985; Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella . Syst Appl Microbiol6:171–182[CrossRef]
    [Google Scholar]
  25. Makemson J. C., Fulayfil N. R., Landry W., Van Ert L. M., Wimpee C. F., Widder E. A., Case J. F.. 1997; Shewanella woodyi sp. nov., an exclusively respiratory luminous bacterium isolated from the Alboran Sea. Int J Syst Bacteriol47:1034–1039 [CrossRef][PubMed]
    [Google Scholar]
  26. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241[CrossRef]
    [Google Scholar]
  27. Nozue H., Hayashi T., Hashimoto Y., Ezaki T., Hamasaki K., Ohwada K., Terawaki Y.. 1992; Isolation and characterization of Shewanella alga from human clinical specimens and emendation of the description of S. alga simidu et al., 1990, 335. Int J Syst Bacteriol42:628–634 [CrossRef][PubMed]
    [Google Scholar]
  28. Park H. Y., Jeon C. O.. 2013; Shewanella aestuarii sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol63:4683–4690 [CrossRef][PubMed]
    [Google Scholar]
  29. Park S. C., Baik K. S., Kim M. S., Kim D., Seong C. N.. 2009; Shewanella marina sp. nov., isolated from seawater. Int J Syst Evol Microbiol59:1888–1894 [CrossRef][PubMed]
    [Google Scholar]
  30. Saitou N., Nei M.. 1987; The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  31. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  32. Satomi M., Vogel B. F., Gram L., Venkateswaran K.. 2006; Shewanella hafniensis sp. nov. and Shewanella morhuae sp. nov., isolated from marine fish of the Baltic Sea. Int J Syst Evol Microbiol56:243–249 [CrossRef][PubMed]
    [Google Scholar]
  33. Satomi M., Vogel B. F., Venkateswaran K., Gram L.. 2007; Description of Shewanella glacialipiscicola sp. nov. and Shewanella algidipiscicola sp. nov., isolated from marine fish of the Danish Baltic Sea, and proposal that Shewanella affinis is a later heterotypic synonym of Shewanella colwelliana. Int J Syst Evol Microbiol57:347–352 [CrossRef][PubMed]
    [Google Scholar]
  34. Smibert R. M., Krieg N. R.. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp.607–654 Edited by Gerhardt P. Washington, D.C., USA: American Society for Microbiology;
    [Google Scholar]
  35. Sung H. R., Yoon J. H., Ghim S. Y.. 2012; Shewanella dokdonensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol62:1636–1643 [CrossRef][PubMed]
    [Google Scholar]
  36. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128[CrossRef]
    [Google Scholar]
  37. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  38. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882[PubMed][CrossRef]
    [Google Scholar]
  39. Toffin L., Bidault A., Pignet P., Tindall B. J., Slobodkin A., Kato C., Prieur D.. 2004; Shewanella profunda sp. nov., isolated from deep marine sediment of the nankai trough. Int J Syst Evol Microbiol54:1943–1949 [CrossRef][PubMed]
    [Google Scholar]
  40. Venkateswaran K., Moser D. P., Dollhopf M. E., Lies D. P., Saffarini D. A., MacGregor B. J., Ringelberg D. B., White D. C., Nishijima M., other authors. 1999; Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol49 Pt 2:705–724 [CrossRef][PubMed]
    [Google Scholar]
  41. Verma P., Pandey P. K., Gupta A. K., Kim H. J., Baik K. S., Seong C. N., Patole M. S., Shouche Y. S.. 2011; Shewanella indica sp. nov., isolated from sediment of the Arabian Sea. Int J Syst Evol Microbiol61:2058–2064 [CrossRef][PubMed]
    [Google Scholar]
  42. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464[CrossRef]
    [Google Scholar]
  43. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol173:697–703[PubMed]
    [Google Scholar]
  44. Wilson K.. 2001; Preparation of genomic DNA from bacteria: miniprep of bacterial genomic DNA. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.2 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;
    [Google Scholar]
  45. Xu M., Guo J., Cen Y., Zhong X., Cao W., Sun G.. 2005; Shewanella decolorationis sp. nov., a dye-decolorizing bacterium isolated from activated sludge of a waste-water treatment plant. Int J Syst Evol Microbiol55:363–368 [CrossRef][PubMed]
    [Google Scholar]
  46. Yamamoto S., Harayama S.. 1995; PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol61:1104–1109[PubMed]
    [Google Scholar]
  47. Yang S. H., Kwon K. K., Lee H. S., Kim S. J.. 2006; Shewanella spongiae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol56:2879–2882 [CrossRef][PubMed]
    [Google Scholar]
  48. Yoon J. H., Kang K. H., Oh T. K., Park Y. H.. 2004; Shewanella gaetbuli sp. nov., a slight halophile isolated from a tidal flat in korea. Int J Syst Evol Microbiol54:487–491 [CrossRef][PubMed]
    [Google Scholar]
  49. Yoon J. H., Park S., Jung Y. T., Lee J. S.. 2012; Shewanella seohaensis sp. nov., isolated from a tidal flat sediment. Antonie Van Leeuwenhoek102:149–156 [CrossRef][PubMed]
    [Google Scholar]
  50. ZoBell C. E.. 1941; Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res4:42–75
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001014
Loading
/content/journal/ijsem/10.1099/ijsem.0.001014
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error