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Abstract

A Gram-stain-negative and non-motile bacterial strain that formed straight rods and reddish colonies, designated KJ035, was isolated from seawater from the East Sea, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KJ035 belonged to the genus . The most closely related species were A2-91 (96.8 % 16S rRNA gene sequence similarity), A2-50A (96.7 %) and DCY57 (96.0 %). The major fatty acids were iso-C, anteiso-C, C 5 and summed feature 3 (comprising C 6 and/or C 7). The major isoprenoid quinone and polyamine were menaquinone 7 (MK-7) and homospermidine, respectively. The major polar lipids were phosphatidylethanolamine, three unidentified aminophospholipids, three unidentified aminolipids, one unidentified phospholipid and five unidentified polar lipids. The DNA G+C content of the genomic DNA was 61.0 mol%. The results of physiological and biochemical tests allowed the discrimination of the new isolate from its phylogenetic relatives. Strain KJ035 is thus considered to be a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KJ035 (=KCTC 42854=CECT 9069).

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2016-06-10
2020-01-22
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. 1997; Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res25:3389–3402[PubMed][CrossRef]
    [Google Scholar]
  2. Brown A. E.. 2007; Benson’s Microbiological Applications: Laboratory Manual in General Microbiology New York: McGraw-Hill;
    [Google Scholar]
  3. Buczolits S., Denner E. B., Kämpfer P., Busse H. J.. 2006; Proposal of Hymenobacter norwichensis sp. nov., classification of ‘Taxeobacter ocellatus’, ‘Taxeobacter gelupurpurascens’ and ‘Taxeobacter chitinovorans’ as Hymenobacter ocellatus sp. nov., Hymenobacter gelipurpurascens sp. nov. and Hymenobacter chitinivorans sp. nov., respectively, and emended description of the genus Hymenobacter Hirsch et al. 1999. Int J Syst Evol Microbiol56:2071–2078 [CrossRef][PubMed]
    [Google Scholar]
  4. Chang X., Zheng J., Jiang F., Liu P., Kan W., Qu Z., Fang C., Peng F.. 2014; Hymenobacter arcticus sp. nov., isolated from glacial till. Int J Syst Evol Microbiol64:2113–2118 [CrossRef][PubMed]
    [Google Scholar]
  5. Chung A. P., Lopes A., Nobre M. F., Morais P. V.. 2010; Hymenobacter perfusus sp. nov., Hymenobacter flocculans sp. nov. and Hymenobacter metalli sp. nov. three new species isolated from an uranium mine waste water treatment system. Syst Appl Microbiol33:436–443 [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376[PubMed][CrossRef]
    [Google Scholar]
  7. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791[CrossRef]
    [Google Scholar]
  8. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20:406–416 [CrossRef]
    [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C.. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol4:770–773[PubMed][CrossRef]
    [Google Scholar]
  10. Han L., Wu S. J., Qin C. Y., Zhu Y. H., Lu Z. Q., Xie B., Lv J.. 2014; Hymenobacter qilianensis sp. nov., isolated from a subsurface sandstone sediment in the permafrost region of Qilian Mountains, China and emended description of the genus Hymenobacter . Antonie Van Leeuwenhoek105:971–978 [CrossRef][PubMed]
    [Google Scholar]
  11. Hirsch P., Ludwig W., Hethke C., Sittig M., Hoffmann B., Gallikowski C. A.. 1998; Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antarctica soils and sandstone: bacteria of the Cytophaga/Flavobacterium/Bacteroides line of phylogenetic descent. Syst Appl Microbiol21:374–383[PubMed][CrossRef]
    [Google Scholar]
  12. Hoang V. A., Kim Y. J., Nguyen N. L., Yang D. C.. 2013; Hymenobacter ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol63:661–666 [CrossRef][PubMed]
    [Google Scholar]
  13. Hucker G. J.. 1921; A new modification and application of the Gram stain. J Bacteriol6:395–397[PubMed]
    [Google Scholar]
  14. Jin L., Lee H. G., Kim S. G., Lee K. C., Ahn C. Y., Oh H. M.. 2014; Hymenobacter ruber sp. nov., isolated from grass soil. Int J Syst Evol Microbiol64:979–983 [CrossRef][PubMed]
    [Google Scholar]
  15. Kang J. W., Lee J. H., Baik K. S., Lee S. S., Seong C. N.. 2015; Hymenobacter wooponensis sp. nov., isolated from wetland freshwater. Int J Syst Evol Microbiol65:1871–1876 [CrossRef][PubMed]
    [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  17. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120[PubMed][CrossRef]
    [Google Scholar]
  18. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 125–175 Edited by Stackebrandt E., Goodfellow M.. Chichester: Wiley;
    [Google Scholar]
  19. Liu L., Zhou E. M., Jiao J. Y., Manikprabhu D., Ming H., Liu W. H., Hozzein W. N., Shu W. S., Li W. J.. 2015; Hymenobacter latericoloratus sp. nov. and Hymenobacter luteus sp. nov., isolated from freshwater sediment. Antonie Van Leeuwenhoek107:165–172 [CrossRef][PubMed]
    [Google Scholar]
  20. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241[CrossRef]
    [Google Scholar]
  21. Pruesse E., Peplies J., Glöckner F. O.. 2012; SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics28:1823–1829 [CrossRef][PubMed]
    [Google Scholar]
  22. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  23. Sasser M.. 1990; Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  24. Scherer P., Kneifel H.. 1983; Distribution of polyamines in methanogenic bacteria. J Bacteriol154:1315–1322[PubMed]
    [Google Scholar]
  25. Stackebrandt E., Goebel B. M.. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol44:846–849 [CrossRef]
    [Google Scholar]
  26. Subhash Y., Sasikala C., Ramana C.. 2014; Hymenobacter roseus sp. nov., isolated from sand. Int J Syst Evol Microbiol64:4129–4133 [CrossRef][PubMed]
    [Google Scholar]
  27. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464[CrossRef]
    [Google Scholar]
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