1887

Abstract

An aerobic, Gram-stain-negative, oxidase-negative, catalase-positive, non-motile, non-spore-forming, rod-shaped, light pink-pigmented bacterium, designated strain R135, was isolated from soil in Hwaseong, South Korea. Flexirubin-type pigments were absent. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain R135 formed a lineage within the family Sphingobacteriaceae of the phylum Bacteroidetes . It was distinct from various species of the genus Pedobacter , including P. terrae DS-57 (98.13 % sequence similarity), P. alluvionis NWER-II11 (97.76 %), P. suwonensis 15-52 (97.71 %), P. kyungheensis KACC 16221 (97.37 %), P. roseus CG-GP80 (97.24 %), P. soli 15-51 (97.23 %) and P. sandarakinus DS-27 (97.09 %). The major isoprenoid quinone was menaquinone-7 (MK-7), and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), iso-C15 : 0, iso-C17 : 0 3-OH and C16 : 0. The DNA G+C content of strain R135 was 40.4 mol%. Levels of DNA–DNA hybridization similarities between strain R135and other members of the genus Pedobacter ranged from 25 % to 43 %. On the basis of phenotypic, genotypic and phylogenetic analysis, strain R135 represents a novel species of the genus Pedobacter , for which the name Pedobacter humicola sp. nov. is proposed. The type strain is R135 (=KEMB 9005-332=KACC 18452=JCM 31010).

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2016-06-10
2019-10-22
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