An alkaliphilic, filamentous actinomycete, designated EGI 80629T, was isolated from a soil sample of Xinjiang, north-west China. Strain EGI 80629T grew at pH 6.0–11.0 (optimum pH 9.0–10.0) and in the presence of 0–13.0 % NaCl (optimum 3.0–5.0 %). The isolate formed fragmented substrate mycelia, and aerial hyphae with short spore chains with rod-like spores. Whole-cell hydrolysates of the isolate contained ll-diaminopimelic acid as the diagnostic diamino acid, and mannose and rhamnose as diagnostic sugars. The major fatty acids identified were iso-C15 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C17 : 0. The predominant menaquinone was MK-9(H4), while the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two phosphatidylinositol mannosides, five unknown phospholipids, three unknown phosphoglycolipids, one unknown glycolipid, four unknown polar lipids and one unknown aminophospholipid. The G+C content of the genomic DNA was 67.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EGI 80629T clustered with the genus Phytoactinopolyspora. The 16S rRNA gene sequence similarity between strain EGI 80629T and Phytoactinopolyspora endophytica EGI 60009T was 96.8 %. Based on morphological, chemotaxonomic and phylogenetic characteristics, strain EGI 80629T represents a novel species of the genus Phytoactinopolyspora, for which the name Phytoactinopolyspora alkaliphila sp. nov. is proposed. The type strain is EGI 80629T ( = CGMCC 4.7225T = KCTC 39701T).
CollinsM. D., PirouzT., GoodfellowM., MinnikinD. E.1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
GrothI., SchumannP., WeissN., MartinK., RaineyF. A.1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [View Article][PubMed]
KellyK. L.1964Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
MesbahM., PremachandranU., WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
MinnikinD. E., AlshamaonyL., GoodfellowM.1975; Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204 [View Article][PubMed]
MinnikinD. E., O'DonnellA. G., GoodfellowM., AldersonG., AthalyeM., SchaalA., ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
SongL., LiW.-J., WangQ.-L., ChenG.-Z., ZhangY.-S., XuL.-H.2005; Jiangella gansuensis gen. nov., sp. nov., a novel actinomycete from a desert soil in north-west China. Int J Syst Evol Microbiol 55:881–884 [View Article][PubMed]
TangS. K., WangY., ChenY., LouK., CaoL. L., XuL. H., LiW. J.2009; Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella
. Int J Syst Evol Microbiol 59:2025–2031 [View Article][PubMed]
TangS. K., ZhiX. Y., WangY., ShiR., LouK., XuL. H., LiW. J.2011; Haloactinopolyspora alba gen. nov., sp. nov., a halophilic filamentous actinomycete isolated from a salt lake, with proposal of Jiangellaceae fam. nov. and Jiangellineae subord. nov. Int J Syst Evol Microbiol 61:194–200 [View Article][PubMed]
WilliamsS. T., GoodfellowM., AldersonG., WellingtonE. M., SneathP. H., SackinM. J.1983; Numerical classification of Streptomycesand related genera. J Gen Microbiol 129:1743–1813[PubMed]
XuP., LiW. J., TangS. K., ZhangY. Q., ChenG. Z., ChenH. H., XuL. H., JiangC. L.2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [View Article][PubMed]