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Abstract

An alkaliphilic, filamentous actinomycete, designated EGI 80629, was isolated from a soil sample of Xinjiang, north-west China. Strain EGI 80629 grew at pH 6.0–11.0 (optimum pH 9.0–10.0) and in the presence of 0–13.0 % NaCl (optimum 3.0–5.0 %). The isolate formed fragmented substrate mycelia, and aerial hyphae with short spore chains with rod-like spores. Whole-cell hydrolysates of the isolate contained -diaminopimelic acid as the diagnostic diamino acid, and mannose and rhamnose as diagnostic sugars. The major fatty acids identified were iso-C, iso-C, anteiso-C and iso-C. The predominant menaquinone was MK-9(H), while the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two phosphatidylinositol mannosides, five unknown phospholipids, three unknown phosphoglycolipids, one unknown glycolipid, four unknown polar lipids and one unknown aminophospholipid. The G+C content of the genomic DNA was 67.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EGI 80629 clustered with the genus . The 16S rRNA gene sequence similarity between strain EGI 80629 and EGI 60009 was 96.8 %. Based on morphological, chemotaxonomic and phylogenetic characteristics, strain EGI 80629 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 80629 ( = CGMCC 4.7225 = KCTC 39701).

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2016-05-01
2019-10-17
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