1887

Abstract

An endohyphal bacterium (strain B1-EB) living in association with the fungus FMR23-6 I-B1 was isolated from a fungal cell homogenate and studied for its taxonomic allocation. Cells were Gram-stain-negative, rod-shaped, non-spore-forming, non-motile, and negative for oxidase and catalase. Strain B1-EB required cysteine for growth and grew at temperatures between 4 and 35 °C. A comparative analysis of 16S rRNA gene sequences revealed that strain B1-EB forms a distinct clade in the family , encompassing a group of endosymbionts associated with several soil isolates of . The most closely related genus is ‘ Glomeribacter gigasporarum’, an endosymbiont of the arbuscular mycorrhizal fungus . The major cellular fatty acids of strain B1-EB were C, summed feature 3 (Cω7 and Cω6) and summed feature 8 (Cω7 or Cω6). Ubiquinone Q-8 was the only quinone detected. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminophospholipid and two unknown aminolipids. The DNA G+C content was 49.8 mol%. On the basis of phenotypic, chemotaxonomic, and phylogenetic characteristics, strain B1-EB represents a novel genus and novel species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is B1-EB ( = JCM 30646 = LMG 28693 = NBRC 110909).

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2016-05-01
2019-10-18
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