A Gram-stain-negative and non-motile bacterial strain designated 9O-5T was isolated from an abandoned lead–zinc mine in Meizhou, Guangdong Province, southern China. The isolate was orange-pigmented, aerobic, and oxidase- and catalase-positive. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain 9O-5T belongs to the genus Sphingomonas and was closely related to Sphingomonas abaci DSM 15867T (97.6 % similarity), Sphingomonas phyllosphaerae FA2T (96.9 %) and Shingomonas guangdongensis 9NM-8T (96.8 %). Mean DNA–DNA relatedness between strain 9O-5T and S. abaci DSM 15867T was only 47.1 ± 4.9 %. The major fatty acids were C18 : 1ω7c, C14 : 0 2-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). It contained Q-10 as the predominant respiratory quinone and sym-homospermidine as the major polyamine. The polar lipids were sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, five unidentified phospholipids and six unidentified lipids. The genomic DNA G+C content of strain 9O-5T was 69.1 ± 0.1 mol%. Based on the data from this polyphasic taxonomic study, strain 9O-5T should be considered as representing a novel species of the genus Sphingomonas, for which the name Sphingomonas metalli sp. nov. is proposed. The type strain is 9O-5T ( = CGMCC 1.15330T = KCTC 42759T).
ChenH., JoglerM., RohdeM., KlenkH. P., BusseH. J., TindallB. J., SpröerC., OvermannJ.2012; Reclassification and emended description of Caulobacter leidyi as Sphingomonas leidyi comb. nov., and emendation of the genus Sphingomonas
. Int J Syst Evol Microbiol 62:2835–2843 [View Article][PubMed]
CollinsM. D., PirouzT., GoodfellowM., MinnikinD. E.1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
De LeyJ., CattoirH., ReynaertsA.1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
HanS. I., LeeJ. C., OhtaH., WhangK. S.2014; Sphingomonas oligoaromativorans sp. nov., an oligotrophic bacterium isolated from a forest soil. Int J Syst Evol Microbiol 64:1679–1684 [View Article][PubMed]
KimJ. H., KimS. H., KimK. H., LeeP. C.2015; Sphingomonas lacus sp. nov., an astaxanthin-dideoxyglycoside-producing species isolated from soil near a pond. Int J Syst Evol Microbiol 65:2824–2830 [View Article][PubMed]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
MesbahM., PremachandranU., WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
TakeuchiM., HamanaK., HiraishiA.2001; Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium,
Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:1405–1417 [View Article][PubMed]
TindallB. J., SikorskiJ., SmibertR. A., KriegN. R.2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology pp 330–393Edited byReddyC. A., BeveridgeT. J., BreznakJ. A., MarzlufG., SchmidtT. M., SnyderL. R. Washington, DC: American Society for Microbiology;
WayneL. G., BrennerD. J., ColwellR. R., GrimontP.A.D., KandlerO., KrichevskyM. I., MooreL. H., MooreW.E.C., MurrayR.G.E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
YabuuchiE., KosakoY., FujiwaraN., NakaT., MatsunagaI., OguraH., KobayashiK.2002; Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, Sphingobium,
Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola
. Int J Syst Evol Microbiol 52:1485–1496[PubMed]