1887

Abstract

A novel sulfur-oxidizing bacterium designated strain mst6 was isolated from spring water of Masutomi hot spring in Japan. The cells were rod-shaped (1.2–4.0 × 0.5–0.7 μm) and Gram-stain-negative. The G+C content of genomic DNA was around 52.6 mol%. The isolate possessed summed feature 3 (Cω7 and/or Cω6), C and C as major cellular fatty acids. Strain mst6 grew by inorganic carbon fixation and oxidation of inorganic sulfur compounds with oxygen as an electron acceptor. The isolate grew over a temperature range of 5–34 °C, a NaCl concentration range of 0–110 mM and a pH range of 4.6–8.1. Optimum growth occurred at 32 °C, in the absence of NaCl and at pH 5.9–6.2. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain mst6 belongs to the family in the class . The closest cultured relative was TTN with a 16S rRNA gene sequence similarity of 97.0 %. On the basis of the data obtained in this study, strain mst6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is mst6 ( = NBRC 111150 = DSM 101871). In addition, we propose correcting the name Watanabe, Kojima and Fukui 2015 to corrig. based on Rule 12c, Rule 61 and Appendix 9 of the International Code of Nomenclature of Prokaryotes.

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2016-05-01
2019-12-13
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References

  1. Drobner E., Huber H., Rachel R., Stetter K. O.. 1992; Thiobacillus plumbophilus spec. nov., a novel galena and hydrogen oxidizer. Arch Microbiol157:213–217 [CrossRef][PubMed]
    [Google Scholar]
  2. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T.. 1984; Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. Agric Biol Chem48:3169–3172 [CrossRef]
    [Google Scholar]
  3. Kojima H., Fukui M.. 2010; Sulfuricella denitrificans gen. nov., sp. nov., a sulfur-oxidizing autotroph isolated from a freshwater lake. Int J Syst Evol Microbiol60:2862–2866 [CrossRef][PubMed]
    [Google Scholar]
  4. Kojima H., Watanabe T., Iwata T., Fukui M.. 2014; Identification of major planktonic sulfur oxidizers in stratified freshwater lake. PLoS One9:e93877 [CrossRef][PubMed]
    [Google Scholar]
  5. Kojima H., Shinohara A., Fukui M.. 2015; Sulfurifustis variabilis gen. nov., sp. nov., a sulfur oxidizer isolated from a lake, and proposal of Acidiferrobacteraceae fam. nov. and Acidiferrobacterales ord. nov. Int J Syst Evol Microbiol65:3709–3713 [CrossRef][PubMed]
    [Google Scholar]
  6. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp115–175Edited by Stackebrandt E., Goodfellow M.. Chichester: Wiley;
    [Google Scholar]
  7. Luo J. F., Lin W. T., Guo Y.. 2011; Functional genes based analysis of sulfur-oxidizing bacteria community in sulfide removing bioreactor. Appl Microbiol Biotechnol90:769–778 [CrossRef][PubMed]
    [Google Scholar]
  8. Meyer B., Kuever J.. 2007a; Molecular analysis of the diversity of sulfate-reducing and sulfur-oxidizing prokaryotes in the environment, using aprA as functional marker gene. Appl Environ Microbiol73:7664–7679 [CrossRef][PubMed]
    [Google Scholar]
  9. Meyer B., Kuever J.. 2007b; Molecular analysis of the distribution and phylogeny of dissimilatory adenosine-5′-phosphosulfate reductase-encoding genes (aprBA) among sulfur-oxidizing prokaryotes. Microbiology153:3478–3498 [CrossRef][PubMed]
    [Google Scholar]
  10. Meyer B., Imhoff J. F., Kuever J.. 2007; Molecular analysis of the distribution and phylogeny of the soxB gene among sulfur-oxidizing bacteria - evolution of the Sox sulfur oxidation enzyme system. Environ Microbiol9:2957–2977 [CrossRef][PubMed]
    [Google Scholar]
  11. Stackebrandt E., Ebers J.. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today33:152–155
    [Google Scholar]
  12. Watanabe T., Kojima H., Takano Y., Fukui M.. 2013; Diversity of sulfur-cycle prokaryotes in freshwater lake sediments investigated using aprA as the functional marker gene. Syst Appl Microbiol36:436–443 [CrossRef][PubMed]
    [Google Scholar]
  13. Watanabe T., Kojima H., Fukui M.. 2014; Complete genomes of freshwater sulfur oxidizers Sulfuricella denitrificans skB26 and Sulfuritalea hydrogenivorans sk43H: genetic insights into the sulfur oxidation pathway of betaproteobacteria. Syst Appl Microbiol37:387–395 [CrossRef][PubMed]
    [Google Scholar]
  14. Watanabe T., Kojima H., Fukui M.. 2015; Sulfuriferula multivorans gen. nov., sp. nov., isolated from a freshwater lake, reclassification of ‘Thiobacillus plumbophilus’ as Sulfuriferula plumbophilus sp. nov., and description of Sulfuricellaceae fam. nov. and Sulfuricellales ord. nov. Int J Syst Evol Microbiol65:1504–1508 [CrossRef][PubMed]
    [Google Scholar]
  15. Watanabe T., Kojima H., Shinohara A., Fukui M.. 2016; Sulfurirhabdus autotrophica gen. nov., sp. nov., isolated from a freshwater lake. Int J Syst Evol Microbiol66:113–117 [CrossRef][PubMed]
    [Google Scholar]
  16. Widdel F., Bak F.. 1992; Gram-negative mesotrophic sulfate- reducing bacteria. In The Prokaryotes, 2nd edn.vol. 4 pp3352–3378Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H.. New York: Springer; [CrossRef]
    [Google Scholar]
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