A novel Gram-stain-positive, coccus-shaped, obligately anaerobic bacterium was isolated from a faecal sample obtained from an individual in a traditional community located off the southern coast of Peru. Comparative 16S rRNA gene sequence analysis showed the novel bacterium belonged to the genus Peptoniphilus but showed no particular relationship with any species, demonstrating less than 91 % 16S rRNA gene sequence similarity with all members of the genus. The major cellular fatty acids of the novel isolate were determined to be C10 : 0, C14 : 0, C16 : 0, C18 : 1ω9c and C18 : 2ω6,9c/anteiso-C18 : 0. The DNA G+C content was 34.4 mol%. End-products of metabolism from peptone-yeast-glucose broth (PYG) were determined to be acetate and butyrate. Based on the phenotypic, chemotaxonomic and phylogenetic results, the organism represents a novel species of the genus Peptoniphilus, for which the name Peptoniphilus catoniae sp. nov. is proposed. The type strain is M6.X2DT ( = DSM 29874T = CCUG 66798T).
BlaserM. J.,
Dominguez-BelloM. G.,
ContrerasM.,
MagrisM.,
HidalgoG.,
EstradaI.,
GaoZ.,
ClementeJ. C.,
CostelloE. K.,
KnightR.2013; Distinct cutaneous bacterial assemblages in a sampling of South American Amerindians and US residents. ISME J 7:85–95 [View Article][PubMed]
ContrerasM.,
CostelloE. K.,
HidalgoG.,
MagrisM.,
KnightR.,
Dominguez-BelloM. G.2010; The bacterial microbiota in the oral mucosa of rural Amerindians. Microbiology 156:3282–3287 [View Article][PubMed]
EckburgP. B.,
BikE. M.,
BernsteinC. N.,
PurdomE.,
DethlefsenL.,
SargentM.,
GillS. R.,
NelsonK. E.,
RelmanD. A.2005; Diversity of the human intestinal microbial flora. Science 308:1635–1638 [View Article][PubMed]
EzakiT.,
KawamuraY.2009; Genus VI. Peptoniphilus
. In Bergey's Manual of Systematic Bacteriologyvol. 3 pp 1136–1137Edited byde VosP.,
GarrityG. M.,
JonesD.,
KriegN. R.,
LudwigW.,
RaineyF. A.,
SchleiferK.-H.,
WhitmanW. B.
New York: Springer;
JohnsonC. N.,
WhiteheadT. R.,
CottaM. A.,
RhoadesR. E.,
LawsonP. A.2014; Peptoniphilus stercorisuis sp. nov., isolated from a swine manure storage tank and description of Peptoniphilaceae fam. nov. Int J Syst Evol Microbiol 64:3538–3545 [View Article][PubMed]
KämpferP.,
KroppenstedtR. M.1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
KoranskyJ. R.,
AllenS. D.,
DowellV.R.,Jr..
1978; Use of ethanol for selective isolation of sporeforming microorganisms. Appl Environ Microbiol 35:762–765[PubMed]
PatelN. B.,
TitoR. Y.,
Obregón-TitoA. J.,
O'NealL.,
Trujillo-VillaroelO.,
Marin-ReyesL.,
Troncoso-CorzoL.,
Guija-PomaE.,
HamadaM.,
other authors.
2015; Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru. Anaerobe 32:43–48 [View Article][PubMed]
ThompsonJ. D.,
HigginsD. G.,
GibsonT. J.1994; clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
TindallB. J.,
SikorskiJ.,
SmibertR. A.,
KriegN. R.2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp 330–393Edited byReddyC. A.,
BeveridgeT. J.,
BreznakJ. A.,
MarzlufG. A.,
SchmidtT. M.,
SnyderL. R.
Washington, DC: American Society for Microbiology; [View Article]
TurnbaughP. J.,
LeyR. E.,
HamadyM.,
Fraser-LiggettC. M.,
KnightR.,
GordonJ. I.2007; The human microbiome project. Nature 449:804–810 [View Article][PubMed]
ZhouY.,
MihindukulasuriyaK. A.,
GaoH.,
La RosaP. S.,
WylieK. M.,
MartinJ. C.,
KotaK.,
ShannonW. D.,
MitrevaM.,
other authors.
2014; Exploration of bacterial community classes in major human habitats. Genome Biol 15:R66 [View Article][PubMed]