1887

Abstract

A Gram-stain-negative, motile, rod-shaped bacterial strain, designated UST20140214-052, was isolated from a marine flatworm (polyclad). The bacterium was found to be catalase-positive and weakly oxidase-positive, and motile by means of several peritrichous or subpolar flagella. Strain UST20140214-052 grew optimally at 28 °C, at pH 7.0 and in the presence of 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain UST20140214-052 belongs to the genus , with highest sequence similarity to UST20140214-015B (98.8 %), followed by WSF2 (96.2 %), F423 (96.2 %), DN34 (95.9 %), Ad2 (95.9 %). All the other species shared < 95.5 % sequence similarity. The DNA–DNA hybridization estimate value between strain UST20140214-052 and UST20140214-015B was 24.7 ± 2.4 %. The major fatty acid was summed feature 8 (Cω7 and/or Cω6, as defined by the MIDI system; 62.6 %). The DNA G+C content was 47.0 mol%. The combined genotypic and phenotypic data show that strain UST20140214-052 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain UST20140214-052 ( = KCTC 42384 = MCCC 1K00452).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000984
2016-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/5/2025.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000984&mimeType=html&fmt=ahah

References

  1. Auch A. F., Klenk H. P., Göker M. 2010a; Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2:142–148 [View Article][PubMed]
    [Google Scholar]
  2. Auch A. F., von Jan M., Klenk H. P., Göker M. 2010b; Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2:117–134 [View Article][PubMed]
    [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.editors 2002 Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 5th edn. New York: Wiley;
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  5. Fukunaga Y., Kurahashi M., Tanaka K., Yanagi K., Yokota A., Harayama S. 2006; Pseudovibrio ascidiaceicola sp. nov., isolated from ascidians (sea squirts). Int J Syst Evol Microbiol 56:343–347 [View Article][PubMed]
    [Google Scholar]
  6. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M. 2007; DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57:81–91 [View Article][PubMed]
    [Google Scholar]
  7. Hosoya S., Yokota A. 2007; Pseudovibrio japonicus sp. nov., isolated from coastal seawater in Japan. Int J Syst Evol Microbiol 57:1952–1955 [View Article][PubMed]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  9. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acids Techniques in Bacterial Systematics pp 115–175Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  10. Luo R., Liu B., Xie Y., Li Z., Huang W., Yuan J., He G., Chen Y., Pan Q., other authors. 2012; SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience 1:18 [View Article][PubMed]
    [Google Scholar]
  11. Meier-Kolthoff J. P., Auch A. F., Klenk H. P., Göker M. 2013; Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14:60 [View Article][PubMed]
    [Google Scholar]
  12. O'Halloran J. A., Barbosa T. M., Morrissey J. P., Kennedy J., Dobson A. D., O'Gara F. 2013; Pseudovibrio axinellae sp. nov., isolated from an Irish marine sponge. Int J Syst Evol Microbiol 63:141–145 [View Article][PubMed]
    [Google Scholar]
  13. Richter M., Rosselló-Móra R. 2009; Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  14. Rzhetsky A., Nei M. 1992; Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 35:367–375 [View Article][PubMed]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  16. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI;
    [Google Scholar]
  17. Shieh W. Y., Lin Y. T., Jean W. D. 2004; Pseudovibrio denitrificans gen. nov., sp. nov., a marine, facultatively anaerobic, fermentative bacterium capable of denitrification. Int J Syst Evol Microbiol 54:2307–2312 [View Article][PubMed]
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P.A.D., Kandler O., Krichevsky M. I., Moore L. H., Moore W.E.C., Murray R.G.E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  20. Xu Y., Li Q., Tian R., Lai Q., Zhang Y. 2015; Pseudovibrio hongkongensis sp. nov., isolated from a marine flatworm. Antonie van Leeuwenhoek 108:127–132 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000984
Loading
/content/journal/ijsem/10.1099/ijsem.0.000984
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error