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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 5

sp. nov., an endophytic actinobacterium isolated from the root of the living fossil L. Free

Abstract

A Gram-stain-positive, aerobic and yellow actinobacterial strain, designated SYP-A7303, was isolated from the root of L. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SYP-A7303 belongs to the genus . The 16S rRNA gene sequence of strain SYP-A7303 showed highest similarity to CL-DD14 ( = JCM 15615) (98.3 %) and GW-9 ( = JCM 11813) (97.1 %), and less than 96.9 % to the type strains of other species of the genus . Strain SYP-A7303 grew optimally at 28 °C, pH 7.0 and in the absence of NaCl. It contained -2,6-diaminopimelic acid in the cell-wall peptidoglycan, with mannose, ribose, rhamnose, glucose and galactose as whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown lipid. The menaquinone was MK-8(H) and the predominant cellular fatty acids were iso-C, Cω9 and Cω8. The DNA G+C content was 72 mol%. Mean DNA–DNA relatedness values between strain SYP-A7303 and the closely related strains JCM 15615 and JCM 11813 were 62.5 ± 2.4 and 56.5 ± 3.5 %, respectively. Based on the morphological, physiological, biochemical and chemotaxonomic characteristics presented in this study, strain SYP-A7303 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SYP-A7303 ( = DSM 100492 = KCTC 39594).

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2016-05-01
2024-04-24
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Nocardioides ginkgobilobae sp. nov., an endophytic actinobacterium isolated from the root of the living fossil Ginkgo biloba L.
Int J Syst Evol Microbiol 66, 2013 (2016); https://doi.org/10.1099/ijsem.0.000983
/content/journal/ijsem/10.1099/ijsem.0.000983
/content/journal/ijsem/10.1099/ijsem.0.000983
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