1887

Abstract

Two novel actinobacteria, designated strains Gsoil 097 and Gsoil 818, isolated from soil of a ginseng field, South Korea, were characterized by a polyphasic approach to clarify their taxonomic positions. They were Gram-reaction-positive, aerobic, non-spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that both isolates belong to the genus and were related most closely to KIS18-7 (99.1 and 98.3 % similarity, respectively), JOS5-1 (97.9 and 97.9 %), Sco-D01 (97.8 and 97.1 %) and SST-45 (97.5 and 97.0 %). The G+C content of the genomic DNA was 68.8 and 70.0 mol%, respectively. Both strains were characterized chemotaxonomically as having -2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H) as the predominant menaquinone and Cω6, Cω9, C 10-methyl and iso-C as major fatty acids. These chemotaxonomic data supported the affiliation of both strains to the genus . However, levels of DNA–DNA relatedness between the two strains and closely related type strains of species were less than 30 %. Moreover, the results of physiological and biochemical tests allowed the phenotypic differentiation of strains Gsoil 097 and Gsoil 818 from other species with validly published names. Therefore, the two isolates represent two novel species, for which the names sp. nov. (type strain Gsoil 097 = KACC 14267 = DSM 22772) and sp. nov. (type strain Gsoil 818 = KACC 14275 = DSM 22773) are proposed.

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2016-05-01
2019-10-15
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