1887

Abstract

A novel thermophilic actinomycete, designated strain T3, was isolated from a soil sample of a sugar cane field. The strain grew at 25–60 °C (optimum 37–50 °C), at pH 6.0–11.0 (optimum 7.0–9.0) and with 0–12.0 % (w/v) NaCl (optimum 0–7 %). The aerial mycelium was white and the vegetative mycelium was colourless to pale yellow. The substrate mycelium fragmented into rod-shaped elements after 4–5 days at 50 °C. The aerial mycelium formed flexuous chains of 5–20 spores per chain; the oval-shaped spores had spiny surfaces and were non-motile. The organism contained -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars consisted of arabinose, galactose and ribose. The cellular fatty acid profile consisted mainly of anteiso-C, iso-C and iso-C. The quinone system was composed predominantly of MK-9(H). The phospholipids detected were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylmethylethanolamine and ninhydrin-positive glycophospholipids. The DNA G+C content of strain T3 was 71.3 mol%. The organism showed a combination of morphological and chemotaxonomic properties typical of members of the genus . In the 16S rRNA gene tree of it formed a distinct phyletic line and was related most closely to 216. However, the phenotypic characteristics of strain T3 were significantly different from those of 216 and DNA–DNA hybridization revealed a low level of relatedness (28.6–32.3 %) between them. Based on the phenotypic and phylogenetic data, strain T3 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is T3 ( = DSM 46801 = CGMCC 4.7206).

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2016-05-01
2019-10-18
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