1887

Abstract

A novel sulfur oxidizer, strain wk12, was isolated from an industrially synthesized lead (II) sulfide. The G+C content of the genomic DNA was around 58.5 mol%. The major components in the cellular fatty acid profile were summed feature 3 (Cω7 and/or Cω6), C and summed feature 8 (Cω7 and/or Cω6). The strain oxidized lead sulfide, thiosulfate and tetrathionate as electron donors to support autotrophic growth. Cells of strain wk12 were motile, rod-shaped (0.5–1.0 × 0.7–2.2 μm), and Gram-stain-negative. For growth, the temperature range was 5–37 °C, and optimum growth was observed at 28–32 °C. The pH range for growth was 5.8–8.7, with optimum growth at pH 6.4–7.1. Optimum growth of the isolate was observed in medium without NaCl, and no growth was observed in the medium containing 0.5 M or more NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the class . The closest relative with a validly published name was DSM 3134, with a 16S rRNA gene sequence similarity of 96 %. On the basis of phylogenetic and phenotypic properties, strain wk12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is wk12 ( = NBRC 111508 = DSM 101799).

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2016-05-01
2020-01-17
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