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Abstract

An actinomycete strain, RY45-3, isolated from a peat swamp forest soil in Rayong Province, Thailand, was characterized using a polyphasic approach. The strain belonged to the genus on the basis of morphological, physiological, biochemical and chemotaxonomic properties. Cell-wall peptidoglycan contained -diaminopimelic acid. The -acyl group of muramic acid in the cell wall was glycolyl type. The diagnostic sugars in whole-cell hydrolysates were galactose and arabinose. MK-8 (Hω-cycl) was the major menaquinone. The major fatty acids were C and Cω9. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The genomic DNA G+C content was 71 mol%. On the basis of 16S rRNA gene sequence similarity analysis, strain RY45-3 was closely related to JCM 12861 (98.9 %), JCM 6044 (98.8 %) and JCM 9894 (98.6 %). The strain showed low levels of DNA–DNA relatedness with JCM 12861, JCM 6044 and JCM 9894 (range from 3.6 to 55.3 %). On the basis of the phenotypic characteristics and the results mentioned, this strain could be differentiated from closely related type strains and represents a novel species of the genus , for which the name sp. nov. (type strain RY45-3 = JCM 19832 = TISTR 2213 = PCU 334) is proposed.

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2016-05-01
2021-10-17
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