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Abstract

A Gram-staining-positive, aerobic, coccoid-shaped, non-motile actinobacterium, designated strain GY0594, was isolated from deep seawater of the western Pacific. Phylogenetic analyses based on 16S rRNA gene sequences showed that this strain was affiliated with the genus with low 16S rRNA gene sequence similarities ( ≤ 96.0 %) with members of the genus Chemotaxonomic characterization of strain GYP0594 supported the result of the phylogenetic analysis. The diagnostic diamino acid in the cell-wall peptidoglycan was -2,6-diaminopimelic acid. The major menaquinone was MK-8(H4). The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unidentified lipid and six unidentified phospholipids. The major cellular fatty acids were iso-C and Cω9. The DNA G+C content of strain GY0594 was determined to be 71.2 mol%. However, strain GY0594 could be distinguished from closely related species by cell morphology, nitrate reduction, aesculin hydrolysis, activity of urease, cystine arylamidase, trypsin and acid phosphatase, assimilation of -acetylglucosamine, maltose, adipic acid, malic acid and phenylacetate, and significant differences in the proportions of several fatty acids. In conclusion, based on the data presented, strain GY0594 should be placed in the genus as a representative of a novel species, for which the name sp. nov. is proposed. The type strain is GY0594 ( = MCCC 1A10561 = KCTC 39638).

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2016-05-01
2021-10-22
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