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Abstract

A novel actinomycete, designated strain 1H-HV4, was isolated from the head of Mayr, which was collected from Northeast Agriculture University (Harbin, Heilongjiang, China). Chemotaxonomic properties of this strain were consistent with those of members of the genus . The cell wall contained -diaminopimelic acid and whole-cell sugars were galactose, glucose and arabinose. The predominant menaquinone was MK-8(H). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were identified as C 10-methyl, C, Cω9 and Cω7. Mycolic acids were found to be present. 16S rRNA gene sequence analysis also showed that strain 1H-HV4 was a member of the genus , with the highest sequence similarities to JCM 4826 (97.39 %), JCM 11441 (97.12 %) and JCM 11439 (97.08 %). 16S rRNA gene sequence similarities to type strains of other members of the genus were less than 97 %. However, DNA–DNA relatedness values and phenotypic data demonstrated that strain1H-HV4 was clearly distinguished from all closely related species of the genus . It is concluded that the isolate can be classified as representing a novel species of the genus , for which the name is proposed. The type strain is 1H-HV4 ( = DSM 100526 = CGMCC 4.7278).

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2016-04-01
2020-04-06
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