1887

Abstract

A Gram-stain-negative, mesophilic bacterial strain, designated SBU1, which degrades polycyclic aromatic hydrocarbons was isolated from the sediments of the mangrove forests of Nayband Bay in the Iranian Persian Gulf during a bioremediation experiment. The 16S rRNA gene sequence of strain SBU1 exhibited highest similarities with P73 (98.52 %) and H 14 (97.05 %). Phylogenetic analysis, based on 16S rRNA gene sequences, demonstrated that strain SBU1 fell within a cluster consisting of the type strains of species of the genus and formed a stable clade with P73 in trees generated with three algorithms. The fatty acid profile of strain SBU1 consisted of the major fatty acids Cω7/ω6 and Cω7 11-methyl. The major compounds in the polar lipid profile were one phosphatidylglycerol and four unidentified phospholipids. The quinone system exclusively comprised ubiquinone (Q-10). The DNA G+C content was 60.4 mol%. A combination of phylogenetic analysis, DNA–DNA hybridization estimation, average nucleotide identity results and differential phenotypic and chemotaxonomic characteristics demonstrated that strain SBU1 could be distinguished from its close relatives. Therefore, strain SBU1 is considered to represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is SBU1 ( = MCCC 1A00672 = DSM 100434).

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2016-04-01
2022-01-18
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