1887

Abstract

A novel actinobacterial strain, AD1-86, was isolated from the vaginal fluid of a Korean female and was characterized by a polyphasic approach. The strain was a facultatively anaerobic, Gram-stain-positive, non-spore-forming, non-motile, catalase-positive and oxidase-negative short rod. Colonies were creamy white, of low convexity and 1–2 mm in diameter after growth on DSM 92 agar plates at 37 °C for 2 days. The most closely related strains were DSM 7083 and 6401990 (98.3 and 96.3 % 16S rRNA gene sequence similarity, respectively). The isolate grew optimally at 37 °C and pH 7 in the presence of 0.5 % (w/v) NaCl. The cell-wall peptidoglycan contained -diaminopimelic acid and the cell-wall hydrolysates contained ribose, galactose and glucose. The DNA G+C content was 62.6 mol% and the mean DNA–DNA relatedness value of the isolate to DSM 7083 was 31.1 ± 3.0 % (reciprocal: 48.2 ± 5.3 %). The major cellular fatty acids (>10 %) were anteiso-C, anteiso-C and iso-C, and the menaquinones were MK-9, MK-8 and MK-7. The polar lipid profile of strain AD1-86 consisted of diphosphatidylglycerol, phosphatidylglycerol, two aminolipids and a glycolipid. Data from this polyphasic study indicate that strain AD1-86 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is AD1-86 ( = KCTC 39585 = DSM 100050).

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2016-04-01
2022-06-27
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