1887

Abstract

A Gram-stain-positive, aerobic organism was isolated as an endophyte from the stem tissue of healthy maize ( and investigated in detail for its taxonomic position. On the basis of the 16S rRNA gene sequence analysis, strain JM-601 was shown to be most closely related to (98.3 %), and (98.0 %). The 16S rRNA gene sequence similarity to all other species of the genus was ≤ 98.0 %. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. The major quinone of strain JM-601 was menaquinone MK-8(H). The polar lipid profile revealed the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylcholine and an unidentified phospholipid. The polyamine pattern contained predominantly spermine and moderate amounts of spermidine. In the fatty acid profile, iso-C, Cω8 and 10-methyl C were present in major amounts. All these data support the allocation of the strain to the genus . The results of physiological and biochemical characterization allow in addition a phenotypic differentiation of strain JM-601 from and . Strain JM-601 represents a novel species of the genus , for which we propose the name sp. nov., with JM-601 ( = CCM 8654 = CIP 110980) as the type strain.

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2016-04-01
2020-01-28
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