1887

Abstract

A Gram-stain-positive bacterium, designated T14, was isolated from the gut of Holotrichia oblita larvae and was subjected to a taxonomic study. The isolate was rod-shaped, aerobic, non-motile, non-spore-forming and yellow-pigmented. Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that the isolate is related to the genus Leucobacter. Its closest neighbours were the type strains ‘Leucobacter kyeonggiensis’ F3-P9 (96.8 % 16S rRNA gene sequence similarity), Leucobacter celer NAL101 (96.2 %) and Leucobacter chironomi DSM 19883 (95.5 %). The DNA G+C content of strain T14 was 69.3 mol%, and DNA–DNA hybridization values with closely related strains were < 32 %. The predominant cellular fatty acids were anteiso-C15 : 0 (49.3 %), iso-C16 : 0 (16.4 %) and anteiso-C17 : 0 (16.8 %). The major polar lipids were aminolipid, diphosphatidylglycerol, phosphatidylglycerol, phospholipid, phosphoglycolipid and unidentified glycolipids. The predominant respiratory quinone was MK-11. Based on these phylogenetic and phenotypic results, strain T14 can be clearly distinguished from all of the recognized species of the genus Leucobacter and is considered to represent a novel species of the genus Leucobacter. The name Leucobacter holotrichiae sp. nov. is proposed, with the type strain T14 ( = DSM 28968 = JCM 30245).

Erratum
This article contains a correction applying to the following content:
Description of Leucobacter holotrichiae sp. nov., isolated from the gut of Holotrichia oblita larvae
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2016-04-01
2019-10-15
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References

  1. Collins M. D. , Jones D. . ( 1981;). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45: 316–354 [PubMed].
    [Google Scholar]
  2. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12: 133–142 [CrossRef] [PubMed].
    [Google Scholar]
  3. Groth I. , Schumann P. , Weiss N. , Martin K. , Rainey F. A. . ( 1996;). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46: 234–239 [CrossRef] [PubMed].
    [Google Scholar]
  4. Halpern M. , Shakéd T. , Pukall R. , Schumann P. . ( 2009;). Leucobacter chironomi sp. nov., a chromate-resistant bacterium isolated from a chironomid egg mass. Int J Syst Evol Microbiol 59: 665–670 [CrossRef] [PubMed].
    [Google Scholar]
  5. Hungate R. E. . ( 1969;). A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B: 117–132.[CrossRef]
    [Google Scholar]
  6. Kim H. J. , Lee S. S. . ( 2011;). Leucobacter kyeonggiensis sp. nov., a new species isolated from dye waste water. J Microbiol 49: 1044–1049 [CrossRef] [PubMed].
    [Google Scholar]
  7. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  8. Kumar S. , Tamura K. , Nei M. . ( 2004;). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5: 150–163 [CrossRef] [PubMed].
    [Google Scholar]
  9. Lee J. H. , Lee S. S. . ( 2012;). Leucobacter margaritiformis sp. nov., isolated from bamboo extract. Curr Microbiol 64: 441–448 [CrossRef] [PubMed].
    [Google Scholar]
  10. Marmur J. , Doty P. . ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118 [CrossRef] [PubMed].
    [Google Scholar]
  11. Morais P. V. , Paulo C. , Francisco R. , Branco R. , Paula Chung A. , da Costa M. S. . ( 2006;). Leucobacter luti sp. nov., and Leucobacter alluvii sp. nov., two new species of the genus Leucobacter isolated under chromium stress. Syst Appl Microbiol 29: 414–421 [CrossRef] [PubMed].
    [Google Scholar]
  12. Owen R. J. , Pitcher D. . ( 1985;). Current methods for estimating DNA base composition and levels of DNA-DNA hybridization. . In Chemical Methods in Bacterial Systematics, pp. 67–93. Edited by Goodfellow M. , Minnikin D. E. . London: Academic Press;.
    [Google Scholar]
  13. Reddy C. A. , Beveridge T. J. , Breznak J. A. , Marzluf G. A. , Schmidt T. M. , Snyder L. R. . (editors) ( 2007;). Methods for general and molecular microbiology , 3rd edn. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  14. Schleifer K. H. . ( 1985;). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18: 123–156 [CrossRef].
    [Google Scholar]
  15. Schleifer K. H. , Kandler O. . ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36: 407–477 [PubMed].
    [Google Scholar]
  16. Shin N. R. , Kim M. S. , Jung M. J. , Roh S. W. , Nam Y. D. , Park E. J. , Bae J. W. . ( 2011;). Leucobacter celer sp. nov., isolated from Korean fermented seafood. Int J Syst Evol Microbiol 61: 2353–2357 [CrossRef] [PubMed].
    [Google Scholar]
  17. Sturm G. , Jacobs J. , Spröer C. , Schumann P. , Gescher J. . ( 2011;). Leucobacter chromiiresistens sp. nov., a chromate-resistant strain. Int J Syst Evol Microbiol 61: 956–960 [CrossRef] [PubMed].
    [Google Scholar]
  18. Takeuchi M. , Weiss N. , Schumann P. , Yokota A. . ( 1996;). Leucobacter komagatae gen. nov., sp. nov., a new aerobic Gram-positive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46: 967–971 [CrossRef] [PubMed].
    [Google Scholar]
  19. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680 [CrossRef] [PubMed].
    [Google Scholar]
  20. Tindall B. J. . ( 1990;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13: 128–130 [CrossRef].
    [Google Scholar]
  21. Ue H. . ( 2011;). Leucobacter exalbidus sp. nov., an actinobacterium isolated from a mixed culture from compost. J Gen Appl Microbiol 57: 27–33 [CrossRef] [PubMed].
    [Google Scholar]
  22. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. , other authors . ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  23. Weon H. Y. , Anandham R. , Tamura T. , Hamada M. , Kim S. J. , Kim Y. S. , Suzuki K. , Kwon S. W. . ( 2012;). Leucobacter denitrificans sp. nov., isolated from cow dung. J Microbiol 50: 161–165 [CrossRef] [PubMed].
    [Google Scholar]
  24. Zhu D. , Tanabe S. H. , Xie C. , Honda D. , Sun J. , Ai L. . ( 2014;). Bacillus ligniniphilus sp. nov., an alkaliphilic and halotolerant bacterium isolated from sediments of the South China Sea. Int J Syst Evol Microbiol 64: 1712–1717 [CrossRef] [PubMed].
    [Google Scholar]
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