1887

Abstract

A novel, salt-dependent, non-motile, rod-shaped, Gram-stain-negative and non-endospore-forming bacterium, designated strain Cs16b, was isolated from the rhizosphere of Arthrocnemum macrostachyum, a halophytic plant at the Lebrija marshes (Seville, Spain). Strain Cs16b was catalase- and oxidase-positive, and able to hydrolyse casein. Growth occurred from 15–40 °C, at pH 6.0–10.0 and with 1–6 % (w/v) NaCl. Q-8 was identified as the major ubiquinone and the predominant cellular fatty acids were iso-C15 : 0, iso-C17 : 1 cis8, iso-C11 : 0 3-OH, iso-C17 : 0, C17 : 0 cyclo and iso-C11 : 0.The polar lipids profile consisted of phosphatidylethanolamine, phosphatidylglycerol, two unknown glycophospholipids, an unknown aminoglycophospholipid, an unknown aminophospholipid and an unknown phospholipid. The 16S rRNA gene of strain Cs16b showed 98.1 %, 97.8 %, and 97.6 % sequence similarity with Microbulbifer maritimus CIP 108504, Microbulbifer taiwanensis DSM 24146 and Microbulbifer gwangyangensis JCM 17800, respectively. Based on the phenotypic and genotypic features, it is concluded that strain Cs16b represents a novel species of the genus Microbulbifer, for which the name Microbulbifer rhizosphaerae sp. nov. is proposed. The type strain is Cs16b ( = DSM 28920 = CECT 8799).

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2016-04-01
2019-10-19
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