1887

Abstract

A moderately halophilic bacterium was isolated from a brine sample of a hypersaline lake, Aran-Bidgol, in Iran. The strain, designated J8B, was Gram-stain-positive, endospore-forming, rod-shaped, strictly aerobic, motile and produced cream colonies. Strain J8B grew in NaCl at between 3.0–15.0 % (w/v) (optimally at 7.5 % NaCl, w/v), between pH 6.5–9.0 (optimally at pH 8.0) and between 20–45 °C (optimally at 35 °C). Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain J8B is a member of the genus and most closely related to CL-MP28, SA9 and R-2 (96.9 %, 96.3 % and 96.2 % similarities, respectively). The level of DNA–DNA relatedness between the novel isolate and IBRC-M 10567 was 10 %. The major cellular fatty acids of the isolate were anteiso-C, iso-C and anteiso-C. The polar lipid pattern of strain J8B consisted of phosphatidylglycerol, diphosphatidylglycerol, five phospholipids, two aminolipids and two glycoaminolipids. It contained MK-7 as the predominant menaquinone and -diaminopimelic acid in the cell-wall peptidoglycan. The G+C content of the genomic DNA of this strain was 39.2 mol%. Phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data suggest that this strain represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain J8B ( = IBRC-M 10444 = DSM 23996).

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2016-03-01
2022-01-17
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