1887

Abstract

A novel actinobacterium designated strain MWE-A11 was isolated from the root of wild Artemisia princeps (mugwort). The isolate was aerobic, Gram-stain-positive and short rod-shaped, and the colonies were yellow and circular with entire margin. Strain MWE-A11 grew at 15–37 °C and pH 6.0–8.0. The predominant isoprenoid quinones were MK-11 and MK-10. The predominant fatty acids were anteiso-C15 : 0 and iso-C16 : 0, and the DNA G+C content was 68.8 mol%. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The peptidoglycan contained 2,4-diaminobutyric acid as the diagnostic diamino acid, and the acyl type was glycolyl. Phylogenetic analyses based on 16S rRNA gene sequence comparisons indicated that strain MWE-A11 was affiliated with the family Microbacteriaceae, and was most closely related to the type strains of Humibacter antri (96.4 % 16S rRNA gene sequence similarity), Herbiconiux moechotypicola (96.3 %), Leifsonia soli (96.3 %), Leifsonia lichenia (96.2 %), Leifsonia xyli subsp. cynodontis (96.1 %), Microbacterium testaceum (96.0 %) and Humibacter albus (96.0 %). However, the combination of chemotaxonomic properties clearly distinguished strain MWE-A11 from the related taxa at genus level. Accordingly, Allohumibacter endophyticus gen. nov., sp. nov. is proposed to accommodate a new member of the family Microbacteriaceae. The type strain of the type species is MWE-A11 ( = JCM 19371 = KCTC 29232).

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2016-04-01
2019-10-20
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