sp. nov. and sp. nov., two tremellaceous yeast species from Brazil Free

Abstract

Two yeast species, sp. nov. and sp. nov., in the family Rhynchogastremataceae of the Tremellales are proposed. The two species are related to six species of the genus : , and . The novel species are proposed on the basis of the sequence-based phylogenetic species concept with analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. A total of 16 strains of sp. nov. were obtained from freshwater and bromeliad leaves collected in Brazil. sp. nov. differs by 11, 12, 16, 14, 11 and 13 substitutions in the D1/D2 domain from the related species , and , respectively. Differences of 11 substitutions and 21 or more substitutions in ITS regions were found when the sequences of sp. nov. were compared with and its closest relatives. The type strain of sp. nov. is UFMG-CM-Y374 ( = CBS 13918). sp. nov. is represented by two strains isolated from a flower of sp. and a water sample in Brazil. sp. nov. differs from the related species and by eight substitutions, from and by 11 substitutions, from by 10 substitutions and from by 6 substitutions in the D1/D2 domain, and by 7 substitutions from and more than 19 substitutions in the ITS region from its closest relatives. The type strain of sp. nov. is CBS 8358 (ML 3666 = DBVPG-4492). The MycoBank numbers for sp. nov. and sp. nov. are MB 813594 and MB 814882, respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000945
2016-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/4/1799.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000945&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [View Article][PubMed]
    [Google Scholar]
  2. Araújo F. V., Rosa C. A., Freitas L. F. D., Lachance M. A., Vaughan-Martini A., Mendonça-Hagler L. C., Hagler A. N. 2012; Kazachstania bromeliacearum sp. nov., a yeast species from water tanks of bromeliads. Int J Syst Evol Microbiol 62:1002–1006 [View Article][PubMed]
    [Google Scholar]
  3. Badenes-Pérez F. R., Alfaro-Alpízar M. A., Johnson M. T. 2010; Diversity, ecology and herbivory of hairstreak butterflies (Theclinae) associated with the velvet tree, Miconia calvescens in Costa Rica. J Insect Sci 10:209 [View Article][PubMed]
    [Google Scholar]
  4. Crestani J., Fontes Landell M., Faganello J., Henning Vainstein M., Simpson Vishniac H., Valente P. 2009; Cryptococcus terrestris sp. nov., a tremellaceous, anamorphic yeast phylogenetically related to Cryptococcus flavescens . Int J Syst Evol Microbiol 59:631–636 [View Article][PubMed]
    [Google Scholar]
  5. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. 2000; Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371 [View Article][PubMed]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  7. García-Robledo C., Staines C. L., Kress W. J. 2015; A new species of bromeliad-feeding Cephaloleia Chevrolat (Coleoptera, Chrysomelidae, Cassidinae) from Costa Rica: evidence from DNA barcodes, larval and adult morphology and insect diets. ZooKeys 477:143–155 [View Article][PubMed]
    [Google Scholar]
  8. Hibbett D. S., Binder M., Bischoff J. F., Blackwell M., Cannon P. F., Eriksson O. E., Huhndorf S., James T., Kirk P. M., other authors. 2007; A higher-level phylogenetic classification of the Fungi. Mycol Res 111:509–547 [View Article][PubMed]
    [Google Scholar]
  9. Inácio J., Landell M. F., Valente P., Wang P. H., Wang Y. T., Yang S. H., Manson J. S., Lachance M. A., Rosa C. A., Fonseca A. 2008; Farysizyma gen. nov., an anamorphic genus in the Ustilaginales to accommodate three novel epiphytic basidiomycetous yeast species from America, Europe and Asia. FEMS Yeast Res 8:499–508 [View Article][PubMed]
    [Google Scholar]
  10. Jha S., Dick C. W. 2010; Native bees mediate long-distance pollen dispersal in a shade coffee landscape mosaic. Proc Natl Acad Sci U S A 107:13760–13764 [View Article][PubMed]
    [Google Scholar]
  11. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  12. Kurtzman C. P. 1973; Formation of hyphae and chlamydospores by Cryptococcus laurentii . Mycologia 65:388–395 [View Article][PubMed]
    [Google Scholar]
  13. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371 [View Article][PubMed]
    [Google Scholar]
  14. Kurtzman C. P., Fell F. W., Boekhout T., Robert V. 2011; Methods for isolation, phenotypic characterization and maintenance of yeasts. In The Yeasts, a Taxonomic Study, 5th edn. vol. I pp 87–110Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [View Article]
    [Google Scholar]
  15. Lachance M. A., Bowles J. M., Starmer W. T., Barker J. S. F. 1999; Kodamaea kakaduensis and Candida tolerans, two new ascomycetous yeast species from Australian Hibiscus flowers. Can J Microbiol 45:172–177 [View Article][PubMed]
    [Google Scholar]
  16. Landell M. F., Inácio J., Fonseca A., Vainstein M. H., Valente P. 2009; Cryptococcus bromeliarum sp. nov., an orange-coloured basidiomycetous yeast isolated from bromeliads in Brazil. Int J Syst Evol Microbiol 59:910–913 [View Article][PubMed]
    [Google Scholar]
  17. Landell M. F., Billodre R., Ramos J. P., Leoncini O., Vainstein M. H., Valente P. 2010; Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil. Int J Syst Evol Microbiol 60:244–248 [View Article][PubMed]
    [Google Scholar]
  18. Landell M. F., Brandão L. R., Barbosa A. C., Ramos J. P., Safar S. V., Gomes F. C. O., Sousa F. M., Morais P. B., Broetto L., other authors. 2014; Hannaella pagnoccae sp. nov., a tremellaceous yeast species isolated from plants and soil. Int J Syst Evol Microbiol 64:1970–1977 [View Article][PubMed]
    [Google Scholar]
  19. Landell M. F., Brandão L. R., Safar S. V. B., Gomes F. C. O., Félix C. R., Santos A. R. O., Pagani D. M., Ramos J. P., Broetto L., other authors. 2015; Bullera vrieseae sp. nov., a tremellaceous yeast species isolated from bromeliads. Int J Syst Evol Microbiol 65:2466–2471 [View Article][PubMed]
    [Google Scholar]
  20. Liu X.-Z., Wang Q.-M., Theelen B., Groenewald M., Bai F.-Y., Boekhout T. 2015a; Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses. Stud Mycol 81:1–26 [View Article]
    [Google Scholar]
  21. Liu X.-Z., Wang Q.-M., Göker M., Groenewald M., Kachalkin A. V., Lumbsch H. T., Millanes A. M., Wedin M., Yurkov A. M., other authors. 2015b; Towards an integrated phylogenetic classification of the Tremellomycetes . Stud Mycol 81:85–147 [View Article]
    [Google Scholar]
  22. O'Donnell K. 1993; Fusarium and its near relatives. In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics pp 225–233Edited by Reynolds D. R., Taylor J. W. Wallingford, UK: CAB International;
    [Google Scholar]
  23. Romero G. Q., Vasconcellos-Neto J. 2005; The effects of plant structure on the spatial and microspatial distribution of a bromeliad-living jumping spider (Salticidae). J Anim Ecol 74:12–21 [View Article]
    [Google Scholar]
  24. Ruivo C. C. C., Lachance M. A., Rosa C. A., Bacci M., Jr, Pagnocca F. C. 2005; Candida bromeliacearum sp. nov. and Candida ubatubensis sp. nov., two yeast species isolated from the water tanks of Canistropsis seidelii (Bromeliaceae). Int J Syst Evol Microbiol 55:2213–2217 [View Article][PubMed]
    [Google Scholar]
  25. Safar S. V. B., Gomes F. C. O., Marques A. R., Lachance M. A., Rosa C. A. 2013; Kazachstania rupicola sp. nov., a yeast species isolated from water tanks of a bromeliad in Brazil. Int J Syst Evol Microbiol 63:1165–1168 [View Article][PubMed]
    [Google Scholar]
  26. Scherrer S., Diniz I. R., Morais H. C. 2010; Climate and host plant characteristics effects on lepidopteran caterpillar abundance on Miconia ferruginata DC and Miconia pohliana Cogn (Melastomataceae). Braz J Biol 70:103–109 [View Article][PubMed]
    [Google Scholar]
  27. Sousa F. M. P., Morais P. B., Lachance M.-A., Rosa C. A. 2014; Hagleromyces gen. nov., a yeast genus in the Saccharomycetaceae, and description of Hagleromyces aurorensis sp. nov., isolated from water tanks of bromeliads. Int J Syst Evol Microbiol 64:2915–2919 [View Article][PubMed]
    [Google Scholar]
  28. Sylvester K., Wang Q.-M., James B., Mendez R., Hulfachor A. B., Hittinger C. T. 2015; Temperature and host preferences drive the diversification of Saccharomyces and other yeasts: a survey and the discovery of eight new yeast species. FEMS Yeast Res 15:1–16 [View Article][PubMed]
    [Google Scholar]
  29. Takashima M., Sugita T., Shinoda T., Nakase T. 2003; Three new combinations from the Cryptococcus laurentii complex: Cryptococcus aureus, Cryptococcus carnescens and Cryptococcus peneaus . Int J Syst Evol Microbiol 53:1187–1194 [View Article][PubMed]
    [Google Scholar]
  30. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  31. White T. J., Bruns T., Lee S., Taylor J. 1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications pp 315–322Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. San Diego, CA: Academic Press; [View Article]
    [Google Scholar]
  32. Yurkov A., Guerreiro M. A., Sharma L., Carvalho C., Fonseca Á. 2015; Multigene assessment of the species boundaries and sexual status of the basidiomycetous yeasts Cryptococcus flavescens and C. terrestris (Tremellales). PLoS One 10:e0120400 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000945
Loading
/content/journal/ijsem/10.1099/ijsem.0.000945
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed