1887

Abstract

A Gram-staining-negative, moderately halophilic and strictly aerobic bacterium, designated strain KJ4, was isolated from marine sediment at Gangjin in South Korea. Cells were catalase- and oxidase-positive long rods with gliding motility. Growth of strain KJ4 was observed at 4–37 °C (optimum, 15–25 °C), at pH 6.0–9.0 (optimum, pH 7.0–8.5) and in the presence of 1.0–7.0 % (w/v) NaCl (optimum, 2.0–5.0 %). Menaquinone 6 (MK-6) was the only isoprenoid quinone detected and iso-C, iso-C 3-OH, iso-C G and summed feature 3 (comprising Cω7 and/or Cω6) were the major cellular fatty acids. The polar lipids of strain KJ4 consisted of phosphatidylethanolamine, an unidentified aminophospholipid and five unidentified lipids. The G+C content of the genomic DNA was 38.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KJ4 formed a distinct phylogenetic lineage within the genus Strain KJ4 was most closely related to KMM 3947 with 97.4 % 16S rRNA gene sequence similarity. On the basis of phenotypic, chemotaxonomic and molecular features, strain KJ4 clearly represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KJ4 ( = KACC 16438 = JCM 31154).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000942
2016-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/4/1773.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000942&mimeType=html&fmt=ahah

References

  1. Barbeyron T., Carpentier F., L'haridon S., Schüler M., Michel G., Amann R. 2008; Description of Maribacter forsetii sp. nov., a marine Flavobacteriaceae isolated from North Sea water, and emended description of the genus Maribacter . Int J Syst Evol Microbiol 58:790–797 [View Article][PubMed]
    [Google Scholar]
  2. Bernardet J.-F. 2011; Family I. Flavobacteriaceae Reichenbach 1992. In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol. 4 pp 106–111Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A. New York: Springer;
    [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070[PubMed]
    [Google Scholar]
  4. Cho K. H., Hong S. G., Cho H. H., Lee Y. K., Chun J., Lee H. K. 2008; Maribacter arcticus sp. nov., isolated from Arctic marine sediment. Int J Syst Evol Microbiol 58:1300–1303 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 2002; phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, WA, USA
    [Google Scholar]
  6. Gomori G. 1955; Preparation of buffers for use in enzyme studies. Methods Enzymol 1:138–146 [View Article]
    [Google Scholar]
  7. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [View Article][PubMed]
    [Google Scholar]
  8. Hu J., Yang Q. Q., Ren Y., Zhang W. W., Zheng G., Sun C., Pan J., Zhu X. F., Zhang X. Q., Wu M. 2015; Maribacter thermophilus sp. nov., isolated from an algal bloom in an intertidal zone, and emended description of the genus Maribacter . Int J Syst Evol Microbiol 65:36–41 [View Article][PubMed]
    [Google Scholar]
  9. Jackson S. A., Kennedy J., Morrissey J. P., O'Gara F., Dobson A. D. 2015; Maribacter spongiicola sp. nov. and Maribacter vaceletii sp. nov., isolated from marine sponges, and emended description of the genus Maribacter . Int J Syst Evol Microbiol 65:2097–2103 [View Article][PubMed]
    [Google Scholar]
  10. Jeong S. H., Jin H. M., Lee H. J., Jeon C. O. 2013; Altererythrobacter gangjinensis sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 63:971–976 [View Article][PubMed]
    [Google Scholar]
  11. Jin H. M., Kim J. M., Lee H. J., Madsen E. L., Jeon C. O. 2012; Alteromonas as a key agent of polycyclic aromatic hydrocarbon biodegradation in crude oil-contaminated coastal sediment. Environ Sci Technol 46:7731–7740 [View Article][PubMed]
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  13. Kim M., Oh H. S., Park S. C., Chun J. 2014; Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 64:346–351 [View Article][PubMed]
    [Google Scholar]
  14. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–208 [View Article]
    [Google Scholar]
  15. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
    [Google Scholar]
  16. Lo N., Jin H. M., Jeon C. O. 2013; Maribacter aestuarii sp. nov., isolated from tidal flat sediment, and an emended description of the genus Maribacter . Int J Syst Evol Microbiol 63:3409–3414 [View Article][PubMed]
    [Google Scholar]
  17. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50:1055–1063 [View Article][PubMed]
    [Google Scholar]
  18. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
    [Google Scholar]
  19. Nawrocki E. P., Eddy S. R. 2007; Query-dependent banding (QDB) for faster RNA similarity searches. PLOS Comput Biol 3:e56 [View Article][PubMed]
    [Google Scholar]
  20. Nedashkovskaya O. I., Kim S. B. 2011; Genus XXXII Maribacter Nedashkovskaya, Kim, Han, Lysenko, Rohde, Rhee, Frolova, Falsen, Mikhailov and Bae 2004b, 1021VP . In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol. 4 pp 235–238Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A. New York: Springer;
    [Google Scholar]
  21. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Rohde M., Rhee M. S., Frolova G. M., Falsen E., Mikhailov V. V., Bae K. S. 2004; Maribacter gen. nov., a new member of the family Flavobacteriaceae, isolated from marine habitats, containing the species Maribacter sedimenticola sp. nov., Maribacter aquivivus sp. nov., Maribacter orientalis sp. nov. and Maribacter ulvicola sp. nov. Int J Syst Evol Microbiol 54:1017–1023 [View Article][PubMed]
    [Google Scholar]
  22. Nedashkovskaya O. I., Vancanneyt M., De Vos P., Kim S. B., Lee M. S., Mikhailov V. V. 2007; Maribacter polysiphoniae sp. nov., isolated from a red alga. Int J Syst Evol Microbiol 57:2840–2843 [View Article][PubMed]
    [Google Scholar]
  23. Nedashkovskaya O. I., Kim S. B., Mikhailov V. V. 2010; Maribacter stanieri sp. nov., a marine bacterium of the family Flavobacteriaceae . Int J Syst Evol Microbiol 60:214–218 [View Article][PubMed]
    [Google Scholar]
  24. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  25. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  26. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  27. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  28. Stamatakis A., Ott M., Ludwig T. 2005; RAxML-OMP: an efficient program for phylogenetic inference on SMPs. In Proceedings of the 8th International Conference on Parallel Computing Technologies (PaCT2005), Lecture Notes in Computer Science3506 pp 288–302 Berlin: Springer;
    [Google Scholar]
  29. Tang M., Wang G., Xiang W., Chen C., Wu J., Dai S., Wu H., Li T., Wu H. 2015; Maribacter flavus sp. nov., isolated from a cyanobactrial culture pond. Int J Syst Evol Microbiol 65:3997–4002 [View Article][PubMed]
    [Google Scholar]
  30. Weerawongwiwat V., Kang H., Jung M. Y., Kim W. 2013; Maribacter chungangensis sp. nov., isolated from a green seaweed, and emended descriptions of the genus Maribacter and Maribacter arcticus . Int J Syst Evol Microbiol 63:2553–2558 [View Article][PubMed]
    [Google Scholar]
  31. Yoon J. H., Kang S. J., Lee S. Y., Lee C. H., Oh T. K. 2005; Maribacter dokdonensis sp. nov., isolated from sea water off a Korean island, Dokdo. Int J Syst Evol Microbiol 55:2051–2055 [View Article][PubMed]
    [Google Scholar]
  32. Zhang G. I., Hwang C. Y., Kang S. H., Cho B. C. 2009; Maribacter antarcticus sp. nov., a psychrophilic bacterium isolated from a culture of the Antarctic green alga Pyramimonas gelidicola . Int J Syst Evol Microbiol 59:1455–1459 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000942
Loading
/content/journal/ijsem/10.1099/ijsem.0.000942
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error