1887

Abstract

The actinomycete strain N74, isolated from cave soil, was studied using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain N74 formed a stable, distinct lineage cluster together with NN276 (99.3 % similarity) and JCM 3151 (97.5 %). Strain N74 was observed to produce single spherical spores on aerial mycelium as reported for JCM 3151 and NN276 but different from other known species of the genus , which are characterized by pairs of spores on aerial hyphae. Multilocus sequence analyses based on concatenated partial , , , and 16S rRNA gene sequences showed a clear distinction of strain N74, JCM 3151 and NN276 from other members of the genus , although the chemotaxonomic characteristics of strain N74 were similar to the genus ; the cell wall contained -diaminopimelic acid and the whole-cell hydrolysate contained madurose as the diagnostic sugar. The major menaquinone was MK-9(H). The fatty acid profile contained iso-C. On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain N74 is assigned to a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is N74 ( = BCC 77604 = NBRC 111481). It is also proposed that and be transferred to this genus as comb. nov. (type strain JCM 3151 = NBRC 14179 = DSM 43048) and comb. nov. (type strain NN276 = BCC 41490 = NBRC 107569), respectively.

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2016-04-01
2020-01-17
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