1887

Abstract

Two strains of Gram-stain-negative, chemo-organotrophic, aerobic and halophilic gammaproteobacteria, isolated from within the stem and roots of in salt marshes from the south Atlantic Spanish coast, were found to represent a novel species in the genus through phylogenetic analysis of their 16S rRNA genes and phenotypic characterization. 16S rRNA gene sequences of the two strains shared < 96.2 % similarity with other species, with being the most similar in sequence. They required sodium ions for growth, were able to thrive at low (4 °C) temperatures and at salinities of 12–15 %, were unable to hydrolyse any tested macromolecule except casein, and grew with different monosaccharides, disaccharides, sugar alcohols, organic acids and amino acids. The novel species differed from other species in the use of several sole carbon sources, its temperature and salinity ranges for growth, ion requirements and cellular fatty acid composition, which included C, C and C as major components and C 3-OH, C and C 3-OH as minor components. The name sp. nov. is proposed, with SMJ19 ( = CECT 8886 = KCTC 42958) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000929
2016-04-01
2022-01-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/4/1686.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000929&mimeType=html&fmt=ahah

References

  1. Arahal D. R., Rodrigo-Torres L., Lucena T., Pujalte M. J. 2015; Draft genome sequences of Vibrio renopiscarius strains CECT 8603T and CECT 8604, two marine Gammaproteobacteria isolated from cultured gilthead sea bream (Sparus aurata). Genome Announc 3:e00099-15 [View Article][PubMed]
    [Google Scholar]
  2. Baumann P., Baumann L. 1981; The marine gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes . In The Prokaryotes vol. 2 pp 1302–1331Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schleger H. Heidelberg: Springer;
    [Google Scholar]
  3. Chimetto L. A., Cleenwerck I., Brocchi M., Willems A., De Vos P., Thompson F. L. 2011; Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis . Int J Syst Evol Microbiol 61:1170–1175 [View Article][PubMed]
    [Google Scholar]
  4. Espinosa E., Marco-Noales E., Gómez D., Lucas-Elío P., Ordax M., Garcías-Bonet N., Duarte C. M., Sanchez-Amat A. 2010; Taxonomic study of Marinomonas strains isolated from the seagrass Posidonia oceanica, with descriptions of Marinomonas balearica sp. nov. and Marinomonas pollencensis sp. nov. Int J Syst Evol Microbiol 60:93–98 [View Article][PubMed]
    [Google Scholar]
  5. Euzéby J. P. 1997; List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592 [View Article][PubMed]
    [Google Scholar]
  6. Farmer J. J. III, Hickman-Brenner F. W. 1992; The genera Vibrio and Photobacterium . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications pp 2952–3011Edited by Balows A. New York: Springer;
    [Google Scholar]
  7. Jung Y.-T., Oh T.-K., Yoon J.-H. 2012; Marinomonas hwangdonensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 62:2062–2067 [View Article][PubMed]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  9. Kumari P., Poddar A., Das S. K. 2014; Marinomonas fungiae sp. nov., isolated from the coral Fungia echinata from the Andaman Sea. Int J Syst Evol Microbiol 64:487–494 [View Article][PubMed]
    [Google Scholar]
  10. Lee I., Kim Y. O., Park S.-C., Chun J. 2016; OrthoANI: an improved algorithm and software for calculating 1 average nucleotide identity. Int J Syst Evol Microbiol (in press) [View Article][PubMed]
    [Google Scholar]
  11. Lucas-Elío P., Marco-Noales E., Espinosa E., Ordax M., López M. M., Garcías-Bonet N., Marbà N., Duarte C. M., Sanchez-Amat A. 2011; Marinomonas alcarazii sp. nov., M. rhizomae sp. nov., M. foliarum sp. nov., M. posidonica sp. nov. and M. aquiplantarum sp. nov., isolated from the microbiota of the seagrass Posidonia oceanica . Int J Syst Evol Microbiol 61:2191–2196 [View Article][PubMed]
    [Google Scholar]
  12. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., other authors. 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  13. Macián M. C., Arahal D. R., Garay E., Ludwig W., Schleifer K. H., Pujalte M. J. 2005; Thalassobacter stenotrophicus gen. nov., sp. nov., a novel marine α-proteobacterium isolated from Mediterranean sea water. Int J Syst Evol Microbiol 55:105–110 [View Article][PubMed]
    [Google Scholar]
  14. Meier-Kolthoff J. P., Auch A. F., Klenk H.-P., Göker M. 2013; Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14:60 [View Article][PubMed]
    [Google Scholar]
  15. Mesa J., Mateos-Naranjo E., Caviedes M. A., Redondo-Gómez S., Pajuelo E., Rodríguez-Llorente I. D. 2015; Endophytic cultivable bacteria of the metal bioaccumulator Spartina maritima improve plant growth but not metal uptake in polluted marshes soils. Front Microbiol 6:1450 [View Article][PubMed]
    [Google Scholar]
  16. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI, Inc;
    [Google Scholar]
  17. Satomi M., Fujii T. 2014; The Family Oceanospirillaceae . In The Prokaryotes: Gammaproteobacteria pp 491–527Edited by Rosenberg E. Berlin: Springer;
    [Google Scholar]
  18. Van Landschoot A., De Ley J. 1983; Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas, Marinomonas (gen. nov.) and some other Gram-negative bacteria. J Gen Microbiol 129:3057–3074
    [Google Scholar]
  19. Yarza P., Ludwig W., Euzéby J., Amann R., Schleifer K.-H., Glöckner F. O., Rosselló-Móra R. 2010; Update of the all-species living tree project based on 16S and 23S rRNA sequence analyses. Syst Appl Microbiol 33:291–299 [View Article][PubMed]
    [Google Scholar]
  20. Zhang D.-C., Li H. R., Xin Y. H., Liu H. C., Chen B., Chi Z. M., Zhou P. J., Yu Y. 2008; Marinomonas arctica sp. nov., a psychrotolerant bacterium isolated from the Arctic. Int J Syst Evol Microbiol 58:1715–1718 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000929
Loading
/content/journal/ijsem/10.1099/ijsem.0.000929
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error