RT Journal Article SR Electronic(1) A1 Duangmal, Kannika A1 Suksaard, Paweena A1 Pathom-aree, Wasu A1 Mingma, Ratchanee A1 Matsumoto, Atsuko A1 Takahashi, YokoYR 2016 T1 Actinopolyspora salinaria sp. nov., a halophilic actinomycete isolated from solar saltern soil JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 4 SP 1660 OP 1665 DO https://doi.org/10.1099/ijsem.0.000926 PB Microbiology Society, SN 1466-5034, AB The taxonomic position of the halophilic actinobacterial strain, HS05-03T, isolated from solar saltern soil, was determined using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence of the strain showed that it formed a distinct evolutionary lineage in the genus Actinopolyspora. The organism was most closely related to the type strains of the species Actinopolyspora xinjiangensis (98.0 % similarity), Actinopolyspora righensis (97.9 % similarity), Actinopolyspora lacussalsi (97.9 % similarity) and Actinopolyspora erythraea (97.8 % similarity). The whole-organism hydrolysates contained meso-diaminopimelic acid, arabinose, galactose and ribose. The predominant menaquinones were found to be MK-9(H4) and MK-10(H4). The acyl type of the peptidoglycan was N-acetyl. The diagnostic phospholipid detected was phosphatidylcholine. The predominant cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0. The G+C content of the genomic DNA was 69.9 mol%. DNA–DNA hybridization values between strain HS05-03T and the type strains of the most closely related species were below the 70 % threshold. On the basis of the phenotypic and genotypic data, it is proposed that strain HS05-03T represents a novel species of the genus Actinopolyspora, with the name Actinopolyspora salinaria sp. nov. The type strain is HS05-03T ( = BCC 51286T = NBRC 109078T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000926