1887

Abstract

Two novel agar-degrading, Gram-stain-negative, motile, heterotrophic, facultatively anaerobic and pale yellow-pigmented bacterial strains, designated Z1 and JL1, were isolated from marine algae (Lamouroux) and , respectively. Growth of the isolates was optimal at 28–30 °C, pH 7.0–7.5 and with 2–3 % (w/v) NaCl. Both strains contained Q-8 as the sole respiratory quinone. The major cellular fatty acids in strain Z1 were Cω7, C and summed feature 3 (Cω7 and/or iso-C 2-OH). The predominant polar lipids in strain Z1 were phosphatidylethanolamine, phosphatidylglycerol and an aminolipid. The genomic DNA G+C content of both strains was 45.1 mol%. Strains Z1 and JL1 were closely related, with 99.9 % 16S rRNA gene sequence similarity. The average nucleotide identity (ANI) value between strains Z1 and JL1 was 99.3 %. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains Z1 and JL1 form a distinct phyletic line within the class , with less than 92.3 % similarity to their closest relatives. Based on data from the current polyphasic study, the isolates are proposed to belong to a novel species of a new genus designated gen. nov., sp. nov. The type strain of the type species is Z1 ( = ATCC BAA-2617 = CICC 10859).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000925
2016-03-01
2020-01-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/3/1593.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000925&mimeType=html&fmt=ahah

References

  1. Baek K., Choi A., Cho J. Ch.. 2015; Eionea flava sp. nov., isolated from coastal seawater, and emended description of the genus Eionea. Int J Syst Evol Microbiol65:2975–2979 [CrossRef][PubMed]
    [Google Scholar]
  2. Chen M. H., Sheu S. Y., Arun A. B., Young C. C., Chen C. A., Wang J. T., Chen W. M.. 2011; Pseudoteredinibacter isoporae gen. nov., sp. nov., a marine bacterium isolated from the reef-building coral Isopora palifera. Int J Syst Evol Microbiol61:1887–1893 [CrossRef][PubMed]
    [Google Scholar]
  3. Cheng H., Zhang S., Huo Y. Y., Jiang X. W., Zhang X. Q., Pan J., Zhu X. F., Wu M.. 2015; Gilvimarinus polysaccharolyticus sp. nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus. Int J Syst Evol Microbiol65:562–569 [CrossRef][PubMed]
    [Google Scholar]
  4. Chi W. J., Chang Y. K., Hong S. K.. 2012; Agar degradation by microorganisms and agar-degrading enzymes. Appl Microbiol Biotechnol94:917–930 [CrossRef][PubMed]
    [Google Scholar]
  5. Cowan S. T., Steel K. J.. 1974; Bacterial characters and characterization. In Cowan and Steel's Manual for the Identification of Medical Bacteria, 2nd edn. Revised by S.T. Cowan. Cambridge, UK: Cambridge University Press;
    [Google Scholar]
  6. Distel D. L., Morrill W., MacLaren-Toussaint N., Franks D., Waterbury J.. 2002; Teredinibacter turnerae gen. nov., sp. nov., a dinitrogen-fixing, cellulolytic, endosymbiotic γ-proteobacterium isolated from the gills of wood-boring molluscs (Bivalvia: Teredinidae). Int J Syst Evol Microbiol52:2261–2269 [CrossRef][PubMed]
    [Google Scholar]
  7. Du Z. J., Zhang D. C., Liu S. N., Chen J. X., Tian X. L., Zhang Z. N., Liu H. C., Chen G. J.. 2009; Gilvimarinus chinensis gen. nov., sp. nov., an agar-digesting marine bacterium within the class Gammaproteobacteria isolated from coastal seawater in Qingdao, China. Int J Syst Evol Microbiol59:2987–2990 [CrossRef][PubMed]
    [Google Scholar]
  8. Hiraishi A., Ueda Y., Ishihara J., Mori T.. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol42:457–469 [CrossRef]
    [Google Scholar]
  9. Kim B. C., Kim M. N., Lee K. H., Kim H. S., Min S. R., Shin K. S.. 2011; Gilvimarinus agarilyticus sp. nov., a new agar-degrading bacterium isolated from the seashore of Jeju Island. Antonie van Leeuwenhoek100:67–73 [CrossRef][PubMed]
    [Google Scholar]
  10. Kim B. C., Poo H., Lee K. H., Kim M. N., Park D. S., Oh H. W., Lee J. M., Shin K. S.. 2012a; Simiduia areninigrae sp. nov., an agarolytic bacterium isolated from sea sand. Int J Syst Evol Microbiol62:906–911 [CrossRef][PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H..other authors 2012b; Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  12. Lee J. H., Baik K. S., Kim D., Seong C. N.. 2014; Psychrosphaera aestuarii sp. nov. and Psychrosphaera haliotis sp. nov., isolated from the marine environment, and emended description of the genus Psychrosphaera. Int J Syst Evol Microbiol64:1952–1957 [CrossRef][PubMed]
    [Google Scholar]
  13. Lim J. M., Jeon C. O., Lee J. C., Song S. M., Kim K. Y., Kim C. J.. 2006; Marinimicrobium koreense gen. nov., sp. nov. and Marinimicrobium agarilyticum sp. nov., novel moderately halotolerant bacteria isolated from tidal flat sediment in Korea. Int J Syst Evol Microbiol56:653–657 [CrossRef][PubMed]
    [Google Scholar]
  14. Liu Q. Q., Wang Y., Li J., Du Z. J., Chen G. J.. 2014; Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol64:2204–2209 [CrossRef][PubMed]
    [Google Scholar]
  15. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., other authors. 2004; arb: a software environment for sequence data. Nucleic Acids Res32:1363–1371 [CrossRef][PubMed]
    [Google Scholar]
  16. Park S., Choi W. C., Oh T. K., Yoon J. H.. 2011; Thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand. Int J Syst Evol Microbiol61:2573–2576 [CrossRef][PubMed]
    [Google Scholar]
  17. Park S., Kim S. I., Jung Y. T., Yoon J. H.. 2014a; Simiduia curdlanivorans sp. nov., a curdlan-degrading bacterium isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Simiduia. Int J Syst Evol Microbiol64:3695–3700 [CrossRef][PubMed]
    [Google Scholar]
  18. Park S., Kim S. I., Kang C. H., Park J. M., Yoon J. H.. 2014b; Simiduia aestuariiviva sp. nov., a gammaproteobacterium isolated from a tidal flat sediment. Antonie van Leeuwenhoek106:927–934 [CrossRef][PubMed]
    [Google Scholar]
  19. Pruesse E., Peplies J., Glöckner F. O.. 2012; sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics28:1823–1829 [CrossRef][PubMed]
    [Google Scholar]
  20. Richter M., Rosselló-Móra R.. 2009; Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A106:19126–19131 [CrossRef][PubMed]
    [Google Scholar]
  21. Shieh W. Y., Liu T. Y., Lin S. Y., Jean W. D., Chen J. S.. 2008; Simiduia agarivorans gen. nov., sp. nov., a marine, agarolytic bacterium isolated from shallow coastal water from Keelung, Taiwan. Int J Syst Evol Microbiol58:895–900 [CrossRef][PubMed]
    [Google Scholar]
  22. Smibert R. M., Krieg N. R.. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp607–654Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  23. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  24. Tanaka N., Romanenko L. A., Svetashev V. I., Mikhailov V. V.. 2014; Simiduia litorea sp. nov., isolated from seashore sediments of the Sea of Japan. Int J Syst Evol Microbiol64:2688–2692 [CrossRef][PubMed]
    [Google Scholar]
  25. Teramoto M., Nishijima M.. 2014; Agaribacter marinus gen. nov., sp. nov., an agar-degrading bacterium from surface seawater. Int J Syst Evol Microbiol64:2416–2423 [CrossRef][PubMed]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  27. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R.. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp330–393Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R.. Washington, DC: American Society for Microbiology; [CrossRef]
    [Google Scholar]
  28. Urios L., Intertaglia L., Lesongeur F., Lebaron P.. 2011; Eionea nigra gen. nov., sp. nov., a gammaproteobacterium from the Mediterranean Sea. Int J Syst Evol Microbiol61:1677–1681 [CrossRef][PubMed]
    [Google Scholar]
  29. Xiao D., Zhao F., Lv M., Zhang H., Zhang Y., Huang H., Su P., Zhang Z., Zhang J.. 2012; Rapid identification of microorganisms isolated from throat swab specimens of community-acquired pneumonia patients by two MALDI-TOF MS systems. Diagn Microbiol Infect Dis73:301–307 [CrossRef][PubMed]
    [Google Scholar]
  30. Yarza P., Richter M., Peplies J., Euzéby J., Amann R., Schleifer K.-H., Ludwig W., Glöckner F. O., Rosselló-Móra R.. 2008; The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol31:241–250 [CrossRef][PubMed]
    [Google Scholar]
  31. Yoon J. H., Kang S. J., Jung Y. T., Oh T. K.. 2009; Marinimicrobium locisalis sp. nov., isolated from a marine solar saltern, and emended description of the genus Marinimicrobium. Int J Syst Evol Microbiol59:2260–2263 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000925
Loading
/content/journal/ijsem/10.1099/ijsem.0.000925
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error