1887

Abstract

Isolates LMG 28357 ( = R-53146) and LMG 28623 were obtained from gut samples of Bombus lapidarius bumblebees caught in Ghent, Belgium. They had identical 16S rRNA gene sequences which were 95.7 % identical to that of Apibacter adventoris wkB301, a member of the family Flavobacteriaceae. Both isolates had highly similar matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS and randomly amplified polymorphic DNA (RAPD) profiles. A draft genome sequence was obtained for strain LMG 28357 (Gold ID Gp0108260); its DNA G+C content was 30.4 %, which is within the range reported for members of the family Flavobacteriaceae (27 to 56 mol%) and which is similar to that of the type strain of A. adventoris (29.0 mol%). Whole-cell fatty acid methyl ester analysis of strain LMG 28357 revealed many branched-chain fatty acids, a typical characteristic of bacteria of the family Flavobacteriaceae and a profile that was similar to that reported for A. adventoris wkB301. MK6 was the major respiratory quinone, again conforming to bacteria of the family Flavobacteriaceae. The isolates LMG 28357 and LMG 28623 could be distinguished from A. adventoris strains through their oxidase activity. On the basis of phylogenetic, genotypic and phenotypic data, we propose to classify both isolates as representatives of a novel species of the genus Apibacter, Apibacter mensalis sp. nov., with LMG 28357 ( = DSM 100903 = R-53146) as the type strain.

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2016-04-01
2019-12-09
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References

  1. Aslam Z. , Im W. T. , Kim M. K. , Lee S. T. . ( 2005;). Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant. Int J Syst Evol Microbiol 55: 747–751 [CrossRef] [PubMed].
    [Google Scholar]
  2. Aziz R. K. , Bartels D. , Best A. A. , DeJongh M. , Disz T. , Edwards R. A. , Formsma K. , Gerdes S. , Glass E. M. , other authors . ( 2008;). The rast Server: rapid annotations using subsystems technology. BMC Genomics 9: 75 [CrossRef] [PubMed].
    [Google Scholar]
  3. Bernardet J.-F. . ( 2010;). Family I. Flavobacteriaceae Reichenbach 1992b, 327VP (effective publication: Reichenbach 1989b, 2013.) emend. Bernardet, Segers, Vancanneyt, Berthe, Kersters and Vandamme 1996, 145 emend. Bernardet, Nakagawa and Holmes 2002, 1057. . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol. 4, pp. 106–111. Edited by Krieg N. R. , Ludwig W. , Whitman W. B. , Hedlund B. P. , Paster B. J. , Staley J. T. , Ward N. L. , Brown D. R. , Parte A. . New York: Springer;.
    [Google Scholar]
  4. Bernardet J.-F. , Nakagawa Y. . ( 2006;). An introduction to the family Flavobacteriaceae . . In The Prokaryotes: a Handbook on the Biology of Bacteria , 3rd edn.., vol. 7, pp. 455–480. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K. H. , Stackebrandt E. . New York: Springer;.
    [Google Scholar]
  5. Cariveau D. P. , Elijah Powell J. , Koch H. , Winfree R. , Moran N. A. . ( 2014;). Variation in gut microbial communities and its association with pathogen infection in wild bumble bees (Bombus). ISME J 8: 2369–2379 [CrossRef] [PubMed].
    [Google Scholar]
  6. da Costa M. S. , Albuquerque L. , Nobre M. F. , Wait R. . ( 2011;). The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. Methods Microbiol 38: 197–206 [CrossRef].
    [Google Scholar]
  7. De Bruyne K. , Schillinger U. , Caroline L. , Boehringer B. , Cleenwerck I. , Vancanneyt M. , De Vuyst L. , Franz C. M. , Vandamme P. . ( 2007;). Leuconostoc holzapfelii sp. nov., isolated from Ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of Leuconostoc species. Int J Syst Evol Microbiol 57: 2952–2959 [CrossRef] [PubMed].
    [Google Scholar]
  8. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.[CrossRef]
    [Google Scholar]
  9. Gallai N. , Salles J.-M. , Settele J. , Vaissière B. E. . ( 2009;). Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecol Econ 68: 810–821 [CrossRef].
    [Google Scholar]
  10. Ghyselinck J. , Van Hoorde K. , Hoste B. , Heylen K. , De Vos P. . ( 2011;). Evaluation of MALDI-TOF MS as a tool for high-throughput dereplication. J Microbiol Methods 86: 327–336 [CrossRef] [PubMed].
    [Google Scholar]
  11. Gnerre S. , Maccallum I. , Przybylski D. , Ribeiro F. J. , Burton J. N. , Walker B. J. , Sharpe T. , Hall G. , Shea T. P. , other authors . ( 2011;). High-quality draft assemblies of mammalian genomes from massively parallel sequence data. Proc Natl Acad Sci U S A 108: 1513–1518 [CrossRef] [PubMed].
    [Google Scholar]
  12. Goulson D. , Lye G. C. , Darvill B. . ( 2008;). Decline and conservation of bumble bees. Annu Rev Entomol 53: 191–208 [CrossRef] [PubMed].
    [Google Scholar]
  13. Killer J. , Kopecný J. , Mrázek J. , Rada V. , Benada O. , Koppová I. , Havlík J. , Straka J. . ( 2009;). Bifidobacterium bombi sp. nov., from the bumblebee digestive tract. Int J Syst Evol Microbiol 59: 2020–2024 [CrossRef] [PubMed].
    [Google Scholar]
  14. Killer J. , Kopečný J. , Mrázek J. , Havlík J. , Koppová I. , Benada O. , Rada V. , Kofronˇová O. . ( 2010;). Bombiscardovia coagulans gen. nov., sp. nov., a new member of the family Bifidobacteriaceae isolated from the digestive tract of bumblebees. Syst Appl Microbiol 33: 359–366 [CrossRef] [PubMed].
    [Google Scholar]
  15. Killer J. , Kopečný J. , Mrázek J. , Koppová I. , Havlík J. , Benada O. , Kott T. . ( 2011;). Bifidobacterium actinocoloniiforme sp. nov. and Bifidobacterium bohemicum sp. nov., from the bumblebee digestive tract. Int J Syst Evol Microbiol 61: 1315–1321 [CrossRef] [PubMed].
    [Google Scholar]
  16. Kim M. , Oh H. S. , Park S. C. , Chun J. . ( 2014;). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 64: 346–351 [CrossRef] [PubMed].
    [Google Scholar]
  17. Klein A.-M. , Vaissière B. E. , Cane J. H. , Steffan-Dewenter I. , Cunningham S. A. , Kremen C. , Tscharntke T. . ( 2007;). Importance of pollinators in changing landscapes for world crops. Proc Biol Sci 274: 303–313 [CrossRef] [PubMed].
    [Google Scholar]
  18. Koch H. , Schmid-Hempel P. . ( 2011a;). Socially transmitted gut microbiota protect bumble bees against an intestinal parasite. Proc Natl Acad Sci U S A 108: 19288–19292 [CrossRef] [PubMed].
    [Google Scholar]
  19. Koch H. , Schmid-Hempel P. . ( 2011b;). Bacterial communities in central European bumblebees: low diversity and high specificity. Microb Ecol 62: 121–133 [CrossRef] [PubMed].
    [Google Scholar]
  20. Koch H. , Schmid-Hempel P. . ( 2012;). Gut microbiota instead of host genotype drive the specificity in the interaction of a natural host-parasite system. Ecol Lett 15: 1095–1103 [CrossRef] [PubMed].
    [Google Scholar]
  21. Kwong W. K. , Moran N. A. . ( 2013;). Cultivation and characterization of the gut symbionts of honey bees and bumble bees: description of Snodgrassella alvi gen. nov., sp. nov., a member of the family Neisseriaceae of the Betaproteobacteria, and Gilliamella apicola gen. nov., sp. nov., a member of Orbaceae fam. nov., Orbales ord. nov., a sister taxon to the order ‘Enterobacteriales’ of the Gammaproteobacteria . Int J Syst Evol Microbiol 63: 2008–2018 [CrossRef] [PubMed].
    [Google Scholar]
  22. Kwong W. K. , Moran N. A. . ( 2013;). Apibacter adventoris gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from honey bees. Int J Syst Evol Microbiol. (In Press) [CrossRef].
    [Google Scholar]
  23. MacFaddin J. F. . ( 1980;). Biochemical Tests for Identification of Medical Bacteria., Baltimore, MD: Williams & Wilkins;.
    [Google Scholar]
  24. McCutcheon J. P. , Moran N. A. . ( 2012;). Extreme genome reduction in symbiotic bacteria. Nat Rev Microbiol 10: 13–26 [PubMed].
    [Google Scholar]
  25. Meeus I. , Brown M. J. F. , De Graaf D. C. , Smagghe G. . ( 2011;). Effects of invasive parasites on bumble bee declines. Conserv Biol 25: 662–671 [CrossRef] [PubMed].
    [Google Scholar]
  26. Meeus I. , Parmentier L. , Billiet A. , Maebe K. , Van Nieuwerburgh F. , Deforce D. , Wäckers F. , Vandamme P. , Smagghe G. . ( 2015;). 16S rRNA amplicon sequencing demonstrates that indoor-reared bumblebees (Bombus terrestris) harbor a core subset of bacteria normally associated with the wild host. PLoS One 10: e0125152 [CrossRef] [PubMed].
    [Google Scholar]
  27. Pitcher D. G. , Saunders N. A. , Owen R. J. . ( 1989;). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8: 151–156 [CrossRef].
    [Google Scholar]
  28. Praet J. , Meeus I. , Cnockaert M. , Houf K. , Smagghe G. , Vandamme P. . ( 2015;). Novel lactic acid bacteria isolated from the bumble bee gut: Convivina intestini gen. nov., sp. nov., Lactobacillus bombicola sp. nov., and Weissella bombi sp. nov. Antonie van Leeuwenhoek 107: 1337–1349 [CrossRef] [PubMed].
    [Google Scholar]
  29. Roulston T. H. , Cane J. H. . ( 2000;). Pollen nutritional content and digestibility for animals. Plant Syst Evol 222: 187–209 [CrossRef].
    [Google Scholar]
  30. Schmidt J. O. . ( 1997;). Bee products: chemical composition and application. . In Bee Products–Properties, Applications, and Apitherapy, pp. 15–26 Edited by Mizrahi L. , Lensky Y. . New York: Springer;.
    [Google Scholar]
  31. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. , Arabia S. , Kumar S. . ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729.[CrossRef]
    [Google Scholar]
  32. Williams J. G. K. , Kubelik A. R. , Livak K. J. , Rafalski J. A. , Tingey S. V. . ( 1990;). DNA polymorphisms amplified by arbitrary primers useful as genetic markers are. 18: 6531–6535.
    [Google Scholar]
  33. Zerbino D. R. , Birney E. . ( 2008;). Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18: 821–829 [CrossRef] [PubMed].
    [Google Scholar]
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