sp. nov., isolated from estuary sediment Free

Abstract

A novel species of the genus , represented by strain S2-21, was isolated from an estuary of Asan in South Korea. Cells of strain S2-21 were Gram-stain-negative, aerobic, non-motile rods that were oxidase- and catalase-positive. Growth of strain S2-21 was observed at 15–40 °C (optimum, 25–30 °C), at pH 7.0–8.0 (optimum, pH 7.0) and in the presence of 0–2.0 % (w/v) NaCl (optimum, 0 %). The major cellular fatty acids were iso-C, C 3-OH, iso-C, summed feature 9 (comprising iso-Cω9 and/or C 10-methyl), anteiso-C and iso-C. The only respiratory quinone detected was ubiquinone-8 (Q-8) and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanol and two unknown phospholipids. The G+C content of the genomic DNA was 62.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S2-21 formed a tight phyletic lineage with HO3-R19 within the genus . Strain S2-21 was related most closely to HO3-R19 at 98.1 % 16S rRNA gene sequence similarity and the mean DNA–DNA relatedness value between strain S2-21 and the type strain of was 23.6 ± 2.2 %. On the basis of phenotypic, chemotaxonomic and molecular features, strain S2-21 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S2-21 ( = KACC 18504 = JCM 31129).

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2016-03-01
2024-03-28
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References

  1. Aslam Z., Park J. H., Kim S. W., Jeon C. O., Chung Y. R. 2009; Arenimonas oryziterrae sp. nov., isolated from a field of rice (Oryza sativa L.) managed under a no-tillage regime, and reclassification of Aspromonas composti as Arenimonas composti comb. nov. Int J Syst Evol Microbiol 59:2967–2972 [View Article][PubMed]
    [Google Scholar]
  2. Chen F., Shi Z., Wang G. 2012; Arenimonas metalli sp. nov., isolated from an iron mine. Int J Syst Evol Microbiol 62:1744–1749 [View Article][PubMed]
    [Google Scholar]
  3. Felsenstein J. 2002 phylip (phylogeny inference package) version 3.6a Distributed by the author Department of Genome Sciences, University of Washington; Seattle, USA:
    [Google Scholar]
  4. Gomori G. 1955; Preparation of buffers for use in enzyme studies. Methods Enzymol 1:138–146 [View Article]
    [Google Scholar]
  5. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [View Article][PubMed]
    [Google Scholar]
  6. Huy H., Jin L., Lee Y.-K., Lee K. C., Lee J.-S., Yoon J.-H., Ahn C.-Y., Oh H.-M. 2013; Arenimonas daechungensis sp. nov., isolated from the sediment of a eutrophic reservoir. Int J Syst Evol Microbiol 63:484–489 [View Article][PubMed]
    [Google Scholar]
  7. Jeong S. H., Jin H. M., Lee H. J., Jeon C. O. 2013; Altererythrobacter gangjinensis sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 63:971–976 [View Article][PubMed]
    [Google Scholar]
  8. Jin L., Kim K. K., Im W. T., Yang H. C., Lee S. T. 2007; Aspromonas composti gen. nov., sp. nov., a novel member of the family Xanthomonadaceae . Int J Syst Evol Microbiol 57:1876–1880 [View Article][PubMed]
    [Google Scholar]
  9. Jin L., Kim K. K., An K. G., Oh H. M., Lee S. T. 2012; Arenimonas daejeonensis sp. nov., isolated from compost. Int J Syst Evol Microbiol 62:1674–1678 [View Article][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  11. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–208 [View Article]
    [Google Scholar]
  12. Kwon S. W., Kim B. Y., Weon H. Y., Baek Y. K., Go S. J. 2007; Arenimonas donghaensis gen. nov., sp. nov., isolated from seashore sand. Int J Syst Evol Microbiol 57:954–958 [View Article][PubMed]
    [Google Scholar]
  13. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
    [Google Scholar]
  14. Lee S. H., Shim J. K., Kim J. M., Choi H.-K., Jeon C. O. 2011; Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . Int J Syst Evol Microbiol 61:722–727 [View Article][PubMed]
    [Google Scholar]
  15. Makk J., Homonnay Z. G., Keki Z., Nemes-Barnas K., Marialigeti K., Schumann P., Toth E. M. 2015; Arenimonas subflava sp. nov., isolated from a drinking water network, and emended description of the genus Arenimonas . Int J Syst Evol Microbiol 65:1915–1921 [CrossRef]
    [Google Scholar]
  16. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
    [Google Scholar]
  17. Nawrocki E. P., Eddy S. R. 2007; Query-dependent banding (QDB) for faster RNA similarity searches. PLOS Comput Biol 3:e56 [View Article][PubMed]
    [Google Scholar]
  18. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R., Swings J., other authors. 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047[PubMed]
    [Google Scholar]
  21. Stamatakis A., Ott M., Ludwig T. 2005; RAxML-OMP: an efficient program for phylogenetic inference on SMPs. In Proceedings of 8th International Conference on Parallel Computing Technologies (PaCT2005), Lecture Notes in Computer Science 3506 pp 288–302 Berlin: Springer; [View Article]
    [Google Scholar]
  22. Wang Q., Garrity G. M., Tiedje J. M., Cole J. R. 2007; Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73:5261–5267 [View Article][PubMed]
    [Google Scholar]
  23. Young C. C., Kämpfer P., Ho M. J., Busse H. J., Huber B. E., Arun A. B., Shen F. T., Lai W. A., Rekha P. D. 2007; Arenimonas malthae sp. nov., a gammaproteobacterium isolated from an oil-contaminated site. Int J Syst Evol Microbiol 57:2790–2793 [View Article][PubMed]
    [Google Scholar]
  24. Yuan X., Nogi Y., Tan X., Zhang R. G., Lv J. 2014; Arenimonas maotaiensis sp. nov., isolated from fresh water. Int J Syst Evol Microbiol 64:3994–4000 [View Article][PubMed]
    [Google Scholar]
  25. Zhang S. Y., Xiao W., Xia Y. S., Wang Y. X., Cui X. L., Zhang N. M. 2015; Arenimonas taoyuanensis sp. nov., a novel bacterium isolated from rice-field soil in China. Antonie van Leeuwenhoek 107:1181–1187 [View Article][PubMed]
    [Google Scholar]
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