1887

Abstract

A novel species of the genus , represented by strain S2-21, was isolated from an estuary of Asan in South Korea. Cells of strain S2-21 were Gram-stain-negative, aerobic, non-motile rods that were oxidase- and catalase-positive. Growth of strain S2-21 was observed at 15–40 °C (optimum, 25–30 °C), at pH 7.0–8.0 (optimum, pH 7.0) and in the presence of 0–2.0 % (w/v) NaCl (optimum, 0 %). The major cellular fatty acids were iso-C, C 3-OH, iso-C, summed feature 9 (comprising iso-Cω9 and/or C 10-methyl), anteiso-C and iso-C. The only respiratory quinone detected was ubiquinone-8 (Q-8) and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanol and two unknown phospholipids. The G+C content of the genomic DNA was 62.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S2-21 formed a tight phyletic lineage with HO3-R19 within the genus . Strain S2-21 was related most closely to HO3-R19 at 98.1 % 16S rRNA gene sequence similarity and the mean DNA–DNA relatedness value between strain S2-21 and the type strain of was 23.6 ± 2.2 %. On the basis of phenotypic, chemotaxonomic and molecular features, strain S2-21 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S2-21 ( = KACC 18504 = JCM 31129).

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2016-03-01
2019-10-20
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References

  1. Aslam Z. , Park J. H. , Kim S. W. , Jeon C. O. , Chung Y. R. . ( 2009;). Arenimonas oryziterrae sp. nov., isolated from a field of rice (Oryza sativa L.) managed under a no-tillage regime, and reclassification of Aspromonas composti as Arenimonas composti comb. nov. Int J Syst Evol Microbiol 59: 2967–2972 [CrossRef] [PubMed].
    [Google Scholar]
  2. Chen F. , Shi Z. , Wang G. . ( 2012;). Arenimonas metalli sp. nov., isolated from an iron mine. Int J Syst Evol Microbiol 62: 1744–1749 [CrossRef] [PubMed].
    [Google Scholar]
  3. Felsenstein J. . ( 2002;). phylip (phylogeny inference package) version 3.6a Distributed by the author Department of Genome Sciences, University of Washington; Seattle, USA:.
    [Google Scholar]
  4. Gomori G. . ( 1955;). Preparation of buffers for use in enzyme studies. Methods Enzymol 1: 138–146 [CrossRef].
    [Google Scholar]
  5. Gonzalez J. M. , Saiz-Jimenez C. . ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4: 770–773 [CrossRef] [PubMed].
    [Google Scholar]
  6. Huy H. , Jin L. , Lee Y.-K. , Lee K. C. , Lee J.-S. , Yoon J.-H. , Ahn C.-Y. , Oh H.-M. . ( 2013;). Arenimonas daechungensis sp. nov., isolated from the sediment of a eutrophic reservoir. Int J Syst Evol Microbiol 63: 484–489 [CrossRef] [PubMed].
    [Google Scholar]
  7. Jeong S. H. , Jin H. M. , Lee H. J. , Jeon C. O. . ( 2013;). Altererythrobacter gangjinensis sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 63: 971–976 [CrossRef] [PubMed].
    [Google Scholar]
  8. Jin L. , Kim K. K. , Im W. T. , Yang H. C. , Lee S. T. . ( 2007;). Aspromonas composti gen. nov., sp. nov., a novel member of the family Xanthomonadaceae . Int J Syst Evol Microbiol 57: 1876–1880 [CrossRef] [PubMed].
    [Google Scholar]
  9. Jin L. , Kim K. K. , An K. G. , Oh H. M. , Lee S. T. . ( 2012;). Arenimonas daejeonensis sp. nov., isolated from compost. Int J Syst Evol Microbiol 62: 1674–1678 [CrossRef] [PubMed].
    [Google Scholar]
  10. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  11. Komagata K. , Suzuki K. . ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19: 161–208 [CrossRef].
    [Google Scholar]
  12. Kwon S. W. , Kim B. Y. , Weon H. Y. , Baek Y. K. , Go S. J. . ( 2007;). Arenimonas donghaensis gen. nov., sp. nov., isolated from seashore sand. Int J Syst Evol Microbiol 57: 954–958 [CrossRef] [PubMed].
    [Google Scholar]
  13. Lányí B. . ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19: 1–67 [CrossRef].
    [Google Scholar]
  14. Lee S. H. , Shim J. K. , Kim J. M. , Choi H.-K. , Jeon C. O. . ( 2011;). Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . Int J Syst Evol Microbiol 61: 722–727 [CrossRef] [PubMed].
    [Google Scholar]
  15. Makk J. , Homonnay Z. G. , Keki Z. , Nemes-Barnas K. , Marialigeti K. , Schumann P. , Toth E. M. . ( 2015;). Arenimonas subflava sp. nov., isolated from a drinking water network, and emended description of the genus Arenimonas . Int J Syst Evol Microbiol 65: 1915–1921.[CrossRef]
    [Google Scholar]
  16. Minnikin D. E. , Patel P. V. , Alshamaony L. , Goodfellow M. . ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27: 104–117 [CrossRef].
    [Google Scholar]
  17. Nawrocki E. P. , Eddy S. R. . ( 2007;). Query-dependent banding (QDB) for faster RNA similarity searches. PLOS Comput Biol 3: e56 [CrossRef] [PubMed].
    [Google Scholar]
  18. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  19. Smibert R. M. , Krieg N. R. . ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  20. Stackebrandt E. , Frederiksen W. , Garrity G. M. , Grimont P. A. D. , Kämpfer P. , Maiden M. C. J. , Nesme X. , Rosselló-Mora R. , Swings J. , other authors . ( 2002;). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52: 1043–1047 [PubMed].
    [Google Scholar]
  21. Stamatakis A. , Ott M. , Ludwig T. . ( 2005;). RAxML-OMP: an efficient program for phylogenetic inference on SMPs. . In Proceedings of 8th International Conference on Parallel Computing Technologies (PaCT2005), Lecture Notes in Computer Science 3506, pp. 288–302 Berlin: Springer; [CrossRef].
    [Google Scholar]
  22. Wang Q. , Garrity G. M. , Tiedje J. M. , Cole J. R. . ( 2007;). Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73: 5261–5267 [CrossRef] [PubMed].
    [Google Scholar]
  23. Young C. C. , Kämpfer P. , Ho M. J. , Busse H. J. , Huber B. E. , Arun A. B. , Shen F. T. , Lai W. A. , Rekha P. D. . ( 2007;). Arenimonas malthae sp. nov., a gammaproteobacterium isolated from an oil-contaminated site. Int J Syst Evol Microbiol 57: 2790–2793 [CrossRef] [PubMed].
    [Google Scholar]
  24. Yuan X. , Nogi Y. , Tan X. , Zhang R. G. , Lv J. . ( 2014;). Arenimonas maotaiensis sp. nov., isolated from fresh water. Int J Syst Evol Microbiol 64: 3994–4000 [CrossRef] [PubMed].
    [Google Scholar]
  25. Zhang S. Y. , Xiao W. , Xia Y. S. , Wang Y. X. , Cui X. L. , Zhang N. M. . ( 2015;). Arenimonas taoyuanensis sp. nov., a novel bacterium isolated from rice-field soil in China. Antonie van Leeuwenhoek 107: 1181–1187 [CrossRef] [PubMed].
    [Google Scholar]
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