gen. nov., sp. nov., a manganese-oxidizing bacterium isolated from deep-sea sediments from the Pacific Polymetallic Nodule Province Free

Abstract

A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped, manganese-oxidizing bacterial strain, designated DY6-4, was isolated from the surface sediment of the Pacific Clarion-Clipperton Fracture Zone. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain DY6-4 formed a lineage within the family and was distinct from the most closely related genera , and (94.0–96.0 %, 93.4–96.0 % and 91.9–95.9 % 16S rRNA gene sequence similarity, repectively). Optimal growth occurred in the presence of 1 % (w/v) NaCl, at pH 7.0 and at 28 °C. Strain DY6-4 contained ubiquinone-10 (Q-10) as the major ubiquinone, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and one unidentified aminolipid as the predominant polar lipids, Cω7 and summed feature 3 (Cω7 and/or Cω6) as the main fatty acids (>10 % of the total). The DNA G+C content of strain DY6-4 was 66.6 mol%. On the basis of the polyphasic analyses, strain DY6-4 is considered to represent a novel species of a novel genus in the clade of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is DY6-4 ( = KCTC 42421 = MCCC 1K00502).

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2016-03-01
2024-03-29
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