1887

Abstract

A novel actinomycete, designated strain S6R2A4-9, was isolated from a soil sample collected from a karst cave in Henan Province, China, and subjected to a polyphasic taxonomic study. This isolate grew optimally at 25–28 °C, pH 6.5–8.0 and in the absence of NaCl. The substrate mycelium of the isolate was well developed with irregular branches. Aerial mycelium fragmented into long, rod-shaped elements. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain S6R2A4-9 resided in the cluster of the genus within the family and shared the highest 16S rRNA gene sequence similarity (98.98 %) with I12A-02601. The G+C content of the genomic DNA was 67.0 mol%. The strain contained glucose, ribose and xylose in its whole-cell hydrolysates. Strain S6R2A4-9 possessed a novel variation of peptidoglycan derived from the type A1γ -Dpm-direct. The polar lipids consisted of diphosphatidylglycerol, -acetylglucosamine-containing phospholipid, phosphatidylinositol mannoside, phosphatidylglycerol, phosphoglycolipids and glycolipids. The predominant menaquinones were MK-10(H) and MK-10(H). The major fatty acids were C, iso-C and 10-methyl C. The level of DNA–DNA relatedness between strain S6R2A4-9 and I12A-02601 was 27.6 ± 3.0 %, which was low enough to indicate that the strain represents a distinct species of the genus . On the basis of the polyphasic taxonomic evidence, a novel species, sp. nov., is proposed. The type strain of the novel species is S6R2A4-9 ( = DSM 28944 = CGMCC 4.7241).

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2016-03-01
2021-10-27
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