RT Journal Article SR Electronic(1) A1 Zhang, Li A1 Ruan, Changying A1 Peng, Fang A1 Deng, Zixin A1 Hong, KuiYR 2016 T1 Streptomyces arcticus sp. nov., isolated from frozen soil JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 3 SP 1482 OP 1487 DO https://doi.org/10.1099/ijsem.0.000907 PB Microbiology Society, SN 1466-5034, AB Actinomycete strain ZLN234T was isolated from a frozen soil sample, which was collected from a glacier front in the Arctic. Chemotaxonomic and morphological characteristics were found to be typical of members of the genus Streptomyces. The strain formed a distinct phyletic line in the 16S rRNA gene tree and was closely related to Streptomyces olivochromogenes ATCC 3336T (98.63 % similarity) and Streptomyces aureus B7319T (98.56 %). Phylogenetic analysis based on the gyrB gene also showed clearly that strain ZLN234T was different from all recognized Streptomyces species. The whole-cell sugars were galactose, glucose, ribose and rhamnose. The whole-cell-wall amino acids included alanine, glutamine, glycine and meso-diaminopimelic acid with a minor amount of ll-diaminopimelic acid. The characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and unknown phospholipids. The major menaquinones were MK-9(H10), MK-9(H8) and MK-9(H4). The major cellular fatty acids comprised anteiso-C15 : 0, iso-C16 : 0 and C16 : 0 with a small amount of iso-C16 : 1ω7 and anteiso-C14 : 0. The DNA G+C content of strain ZLN234T was 77.5 mol%. On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, strain ZLN234T ( = CCTCC AA 2015005T = DSM 100713T) is the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces arcticus sp. nov. is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000907